NOTCH4 elisa kit product blogThe Human NOTCH4 notch4 (Catalog #MBS900186) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS900186 ELISA Kit recognizes Human (General) NOTCH4.
The NOTCH4 notch4 product has the following accession number(s) (GI #55770876) (NCBI Accession #NP_004548.3) (Uniprot Accession #Q99466). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
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(Or you can also download the PDF Manual for complete product instructions).
Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Human Neurogenic locus notch homolog protein 4, NOTCH4 ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for NOTCH4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NOTCH4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NOTCH4 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NOTCH4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Samples: Serum, plasma, tissue homogenates and Cell lysates
Assay Type: Sandwich
Detection Range: 78 pg/ml-5000 pg/ml
Sensitivity: 19.5 pg/ml. Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Detection Wavelength: 450 nm
Sample Volume: 50-100ul
Protein Biological Process 1: Developmental Protein
Protein Biological Process 3: Differentiation. Human Neurogenic locus notch homolog protein 4, NOTCH4 ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit. NOTCH4 also interacts with the following gene(s): CDH5, DLL4, FBXW7, MAML1, MAML2, PSEN1, PSEN2, RBPJ, VEGFA. Brain Diseases, Breast Neoplasms, Cell Transformation, Neoplastic, Disease Models, Animal, Hemorrhage, Inflammation, Liver Diseases, Necrosis, Neoplasms, Experimental, Nervous System Diseases are some of the diseases may be linked to Human Neurogenic locus notch homolog protein 4, NOTCH4 ELISA Kit.