GAD autoantibody (IgG) elisa kit product blogThe Mouse GAD gad2 (Catalog #MBS700640) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS700640 ELISA Kit recognizes Mouse (General) GAD.
The GAD gad2 product has the following accession number(s) (GI #182942) (NCBI Accession #AAA52513.1) (Uniprot Accession #Q05329). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Mouse glutamic acid decarboxylase (GAD) autoantibody, IgG ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with purified GAD antigen. Samples are then added to the appropriate microtiter plate wells and incubated. Then add Horseradish Peroxidase (HRP)-conjugated anti-mouse IgG to each well and incubate. Finally, substrate solution An and B are added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. Calculate the valence of GAD (IgG) in the samples.
Samples: Serum, plasma. Detection Wavelength: 450 nm
Sample Volume: 50-100ul
Protein Biological Process 1: Autoimmunity. Mouse glutamic acid decarboxylase (GAD) autoantibody, IgG ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit. Brain Diseases, Depressive Disorder, Epilepsy, Inflammation, Mental Disorders, Movement Disorders, Nervous System Diseases, Schizophrenia, Seizures, Substance Withdrawal Syndrome are some of the diseases may be linked to Mouse glutamic acid decarboxylase (GAD) autoantibody, IgG ELISA Kit. Brain, Pancreas tissues are correlated with this protein. GAD also interacts with the following gene(s): ABAT, GAD1, GLUL, INS, SLC32A1.