DAB Stable Liquid Substrate 50X Concentrate, 0.0694mM Staining System for HRPO substrate product blogThe DAB Stable Liquid Substrate 50X Concentrate, 0.0694mM Staining System for HRPO n/a (Catalog #MBS639197) is a Substrate and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s DAB Stable Liquid Substrate 50X Concentrate, 0.0694mM Staining System for HRPO can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). Researchers should empirically determine the suitability of the DAB Stable Liquid Substrate 50X Concentrate, 0.0694mM Staining System for HRPO n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
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DAB is widely used for both immunoblotting and immunohistochemical staining techniques. It produces an insoluble end product that is brown in color and insoluble in alcohol. It is supplied as 50X concentrate.
DAB is the most common reagent employed for the immunohistochemical detection of horseradish peroxidase (HRP) labeled probes. DAB powder from different sources may show great variability with respect to purity. Histochemical grade material should be pale tan with no purple hue. The purple material is a DAB oxidation product that binds avidly to heme proteins such as myoglobin, hemoglobin and particularly, catalase. Therefore purple by-products should be avoided for histochemical uses.
MyBioSource provides a stable DAB concentrate manufactured from carefully selected reagents. When diluted in appropriate buffers with added hydrogen peroxide, the resulting solution provides excellent results with most immunohistochemical and blotting procedures.
CAS Number: 91-95-2. Note: DAB is a suspected carcinogen.