APOA2 elisa kit product blogThe Human APOA2 apoa2 (Catalog #MBS704270) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS704270 ELISA Kit recognizes Human (General) APOA2.
The APOA2 apoa2 product has the following accession number(s) (GI #4502149) (NCBI Accession #NP_001634.1) (Uniprot Accession #P02652). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Human Apolipoprotein A2, apo-A2 ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Introduction: Apolipoprotein A2 is a 17.5 kDa protein with a pI of 4.9. Lipids, as such areinsoluble in plasma. They combine with apolipoproteins to form lipoproteinsand this process solubilises lipids in plasma, providing a transport systemfor lipids. Apolipoproteins also play an important role in lipoprotein receptorrecognition and regulation of certain enzymes in lipoprotein metabolism.Two main forms of high density lipoprotein exist; both contain APOA1, butone type only contains APO A2.For more information on APO A2 pleasedownload the PDF catalogue below.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with anantibody specific to apo-A2. Standards or samples are then added to theappropriate microtiter plate wells with a biotin-conjugated polyclonalantibody preparation specific for apo-A2 and Avidin conjugated toHorseradish Peroxidase (HRP) is added to each microplate well andincubated. Then a TMB (3,3\',5,5\' tetramethyl-benzidine) substrate solutionis added to each well. Only those wells that contain apo-A2,biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit achange in color. The enzyme-substrate reaction is terminated by theaddition of a sulphuric acid solution and the color change is measuredspectrophotometrically at a wavelength of 450 nm +/- 2 nm. Theconcentration of apo-A2 in the samples is then determined by comparingthe O.D. of the samples to the standard curve.
Detection Range: 6.2 ug/ml-400 ug/ml?
Sensitivity: 1.6 ug/ml. Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Detection Wavelength: 450 nm
Sample Volume: 50-100ul
Protein Biological Process 1: Immunity
Protein Biological Process 3: Host-virus interaction. Human Apolipoprotein A2, apo-A2 ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.