anti-PPP1CA antibody product blogThe PPP1CA ppp1ca (Catalog #MBS245493) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Monoclonal [clone 4G3] (IgG2a,k) to Human PPP1CA / PP1-Alpha reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s PPP1CA / PP1-Alpha can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA).
ELISA, IHC-P (5 ug/ml), WB (1:500 - 1:2000)
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti.. Researchers should empirically determine the suitability of the PPP1CA ppp1ca for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The PPP1CA ppp1ca product has the following accession number(s) (GI #4506003) (NCBI Accession #NP_002699.1) (Uniprot Accession #P62136). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Mouse Monoclonal [clone 4G3] (IgG2a,k) to Human PPP1CA / PP1-Alpha with the following immunoassay(s):
Immunohistochemistry (IHC) (Anti-PPP1CA antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
Immunohistochemistry (IHC) (Anti-PPP1CA antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
Western Blot (WB) (Cell lysates of HepG2 (45 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human PPP1CA (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)
Protein Phosphatase 1 alpha (PPP1CA), a PP1 type serine/threonine phosphatase, is a catalytic subunit of Protein Phosphatase 1 which has been shown to function in cell division, glycogen metabolism, muscle contractility and protein synthesis. At least two isoforms have been reported. PPP1CA has been implicated in multiple endocrine neoplasia.
Target Species: Human
Immunogen Description: Recombinant human PPP1CA (30-299aa) purified from E Coli. Immunogen Type: Recombinant protein
Immunogen: PPP1CA / PP1-Alpha antibody was raised against recombinant human PPP1CA (30-299aa) purified from E Coli.
Antigen Modification: aa30-299. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing PPP1CA are readily searchable from our website. Different antibodies against the same target such as PPP1CA may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.