anti-mTOR antibody product blogThe mTOR n/a (Catalog #MBS462086) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti mTOR Polyclonal antibody reacts with Human, Rat, Mouse, Bovine, Canine, Chicken and may cross-react with other species as described in the data sheet. MyBioSource\'s mTOR can be used in a range of immunoassay formats including, but not limited to, ELISA, Western Blot, Immunohistochemistry.
Western Blot: 0.1-1 ug/ml
ELISA: 0.01-0.1 ug/ml
Immunoprecipitation: 2-5 ug/ml. Researchers should empirically determine the suitability of the mTOR n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Rabbit anti mTOR Polyclonal antibody with the following immunoassay(s):
The mammalian target of rapamycin (mTOR), also named FKBP12 rapamycin-associated protein (FRAP/RAFT/RAPT/SEP), is a Ser/Thr protein kinase that plays a crucial role in a nutrient-sensitive signalling pathway that regulates cell growth. TOR signalling is potently inhibited by rapamycin, through the direct binding of a FK506-binding protein 12 (FKBP12)/rapamycin complex to the TOR FRB domain, a segment amino terminal to the kinase catalytic domain. mTOR is involved in the regulation of cell growth through initiation of gene translation in response to nutrients such as amino acids (mainly leucine), growth factors, insulin and mitogens. mTOR initiates translation by activating the ribosomal p70S6k protein kinase (S6K1) and by inhibiting the eIF4E inhibitor 4E-BP1. mTOR is phosphorylated at serine 2448 via the phosphatidylinositol 3-kinase (PI3K)/Akt pathway and is autophosphorylated at serine 2481.
Antigen Preparation: A synthetic peptide corresponding to the C-terminus of human mTOR protein. This sequence is identical among human, rat, mouse, chicken, dog and bovine species. Positive Control: MCF-7. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing mTOR are readily searchable from our website. Different antibodies against the same target such as mTOR may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.