anti-IL-8 antibody product blogThe IL-8 il8 (Catalog #MBS551025) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti IL-8 Polyclonal reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s IL-8 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), IHC (Immunohistochemistry), ELISA (EIA).
ELISA (EIA): Use at a concentration range of 1-2 ug/ml.
Western blot (WB): Use at a concentration range of 2-10 ug/mL.
Immunohistochemistry (IHC): A suitable range of concentrations of this antibody for IHC is 2-10 ug/ml. Researchers should empirically determine the suitability of the IL-8 il8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The IL-8 il8 product has the following accession number(s) (GI #10834978) (NCBI Accession #NP_000575.1) (Uniprot Accession #P10145). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Rabbit anti IL-8 Polyclonal with the following immunoassay(s):
Immunohistochemistry (Immunohistochemical analysis of IL-8 expression mouse ovarian vascular endothelial cells.
It was used as primary antibody, dilution 1:200.)
IL8 is produced by stimulated monocytes but not by tissue macrophages and T- lymphocytes. In many cell types the synthesis of IL8 is strongly stimulated by IL1 and TNF- alpha. In human skin fibroblasts the expression of IL-8 is enhanced by Leukoregulin.The expression of IL-8 from resting and stimulated human blood monocytes is up-regulated by IL-7. In chondrocytes the synthesis of IL8 is stimulated by IL1-beta TNF- alpha and bacterial lipopolysaccharides. In human astrocytes the synthesis and secretion of IL8 is induced by IL-1 and TNF-alpha. The IL8 receptor is a dimeric glycoprotein consisting of a 59 kDa and a 67 kDa subunit. It has been given the name CD128. It is expressed in many different cell types including those not responding to IL8. The biological activities of IL8 resemble those of a related protein, NAP-2. IL8 differs from all other cytokines in its ability to specifically activate neutrophil granulocytes. In neutrophils IL8 causes a transient increase in cytosolic calcium levels and the release of enzymes from granules. IL-8 also enhances the metabolism of reactive oxygen species and increases Chemotaxis and the enhanced expression of adhesion molecules. A pre-activation by IL3 is required to render basophils and neutrophils susceptible to further activation by IL8.
Host Note: Polyclonal Interleukin-8 antibody was produced from sera of rabbits immunized with synthetic protein containing Interleukin-8 antigenic determinants.
Dilution: A quick spin of the vial followed by reconstitution in 0.01M PBS pH 7.4 or other diluents.