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anti-EBV/LMP-1 antibody product blog

Tags: Antibody; Monoclonal Antibody; EBV/LMP-1; anti-EBV/LMP-1 antibody;

     The EBV/LMP-1 lmp1 (Catalog #MBS684063) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti - EBV/LMP-1 reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s EBV/LMP-1 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC) - Formalin/Paraffin.
Dilution: 1:100-1:200. Researchers should empirically determine the suitability of the EBV/LMP-1 lmp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The EBV/LMP-1 lmp1 product has the following accession number(s) (GI #126373) (NCBI Accession #P03230.1) (Uniprot Accession #P03230). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Anti - EBV/LMP-1 with the following immunoassay(s):
Test Data (HRS cells of the classical Hodgkin Lymphoma showing cytoplasmic expression of the EBV LMP-1 protein. Formalin fixed, paraffin embedded human tissue (4 Um section) stained with anti - EBV LMP-1 (MBS684063) monospecific clonal antibody according to related DB Biotech datasheet.)
Test Data EBV/LMP-1.

Immunogen: Peptide derived from the C-terminal region of Epstein Barr virus (EBV), Latent Membrane Protein-1 (LMP -1). Antibody recognizes the epitope between Ser369 -Tyr384.
Buffer: 20 mM Tris-HCI, pH 8.0
Stabilizer: 20 mg/ml BSA
Preservative: 0.05% NaN3
Immunogen: Peptide derived from the C-terminal region of Epstein-Barr virus (EBV), Latent Membrane Protein-1 (LMP-1). Antibody recognizes the epitope between Ser369 - Tyr384.
Cellular localization: nucleus
Positive control: lymph node Hodgkin’s lymphoma tissue. IHC-P PROTOCOL: 1.Deparaffinize the section in 3 changes of xylene, 5 minutes each.
2.Wash the section in 96%, 80% and 0% thyl alcohol for 10 minutes each.
3.Rinse in distilled water.
4.Block the endogenous peroxidase by incubating the tissuein 3% hydrogen peroxide (H2O2) for 10 minutes.
5.Wash in distilled water for 5 minutes.
6.For antigen retrieval: immerse the slide in the citrate buffer, pH 6.0, 0.05% Tween-20*, and incubate in ater bath at 96°C for 40 minutes. (Alternatively adjust to your own protocol, keeping the required pH).
7.Remove the staining to room temperature and let the slide to cool (in citrate buffer, pH 6.0) for 20 minutes.
8.Rinse in distilled water.
9.Wash in 0.05 M Tris-HCl, pH 7.6 buffer supplemented with 0.2% of Tween-20 (buffer A) for 5 minutes.
10.CONCENTRATED:Incubate the section with primary antibody at the dilution 1:100 -1:200 for 1 hour in the closed wet chamber.
.READY TO USE (RTU):Incubate the section with primary antibody (ready to use)for 1 hour in a closed wet chamber.
11.Wash twice 5 minutes with buffer A.
12.Apply the secondary antibody (the protocol depends on the supplier), and proceed to standard immunohistochemistry protocol (HRP -Peroxide -DAB). Micropolymer-HRP detection kit rabbit/mouse.
13.Wash twice 5 minutes with buffer A.
14.Apply the chromogen (DAB), 1 -3 minutes.
15.Wash in water for 10 minutes.
16.Stain in hematoxylin for 5 minutes.
17.Wash in water for 10 minutes.
18.Dehydrate the section in 2 changes of 96% ethyl alcohol for 5 minutes each.
19.Wash the section in 2 changes of xylene for 2 minutes each.
20.Mount the slide for observation.

Citrate Buffer(10mM Citric Acid, 0.05% Tween-20, pH 6.0):
Citric acid (anhydrous) ----------1.92 g; Distilled water ---------1000 ml.
Mix to dissolve in 700 ml of distilled water. Adjust pH to 6.0 with 1M NaOH and then add 0.5 ml of Tween-20 and mix well.
Adjust the final volume to 1 liter with distilled water.

Store this solution at room temperature for 3 months or at +4°C for longer storage.
PRECAUTIONS: 1.-We strongly recommend to use Primary Antibody Diluent eventually alternative diluent containing protease free BSA at the concentrations ? 1mg/ml) for dilution of concentrated antibodies, otherwise the warranty might be voided.
2.Centrifuge the vial before use.
3.Intended for professional In Vitro Diagnostic use in laboratories.
4.Do not use after expiration date stamped on vial label.
5.Avoid contamination of the reagent.
6.Any discrepancies in the recommended procedures stated in the working protocol may affect the final results.
7.The reagent contains sodium azide (NaN3) which is highly toxic in higher concentrations. The concentration in the reagent (0.05%) is not considered as hazardous.
8.Disposal of waste material must be conducted in accordance with local regulations.
9.Wear appropriate Personal Protective Equipment to avoid contact with eyes and skin.
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