Anhydrotrypsin, Bovine enzyme product blogThe Anhydrotrypsin, Bovine n/a (Catalog #MBS653795) is an Enzyme and is intended for research purposes only. The product is available for immediate purchase.
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Treatment of trypsin with phenylmethylsulfonyl fluoride (PMSF) followed by a strong base leads to the conversion of Serine 195 to dehydroalanine at the active site (1). Alpha and beta forms of anhydrotrypsin are separated by immobilized soybean trypsin inhibitor. The activity of anhydrotrypsin is greatly reduced compared to native trypsin as quantified by chromogenic substrate (2). Anhydrotrypsin provided by Molecular Innovations is the single chain beta-form prepared from Type XIII bovine trypsin. It has a MW of 23.3kD and an extinction coefficient of 1.54mg-1 ml-1.