ZAP70 blocking peptide product blog
Tags: Blocking Peptide; ZAP70; ZAP70 blocking peptide;
The ZAP70 zap70 (Catalog #MBS9227293) is a Blocking Peptide and is intended for research purposes only. The product is available for immediate purchase.The ZAP70 zap70 product has the following accession number(s) (GI #1177044) (NCBI Accession #P43403.1) (Uniprot Accession #P43403). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
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Tyrosine kinase that plays an essential role in regulation of the adaptive immune response. Regulates motility, adhesion and cytokine expression of mature T-cells, as well as thymocyte development. Contributes also to the development and activation of primary B-lymphocytes. When antigen presenting cells (APC) activate T-cell receptor (TCR), a serie of phosphorylations lead to the recruitment of ZAP70 to the doubly phosphorylated TCR component CD247/CD3Z through ITAM motif at the plasma membrane. This recruitment serves to localization to the stimulated TCR and to relieve its autoinhibited conformation. Release of ZAP70 active conformation is further stabilized by phosphorylation mediated by LCK. Subsequently, ZAP70 phosphorylates at least 2 essential adapter proteins: LAT and LCP2. In turn, a large number of signaling molecules are recruited and ultimately lead to lymphokine production, T-cell proliferation and differentiation. Furthermore, ZAP70 controls cytoskeleton modifications, adhesion and mobility of T-lymphocytes, thus ensuring correct delivery of effectors to the APC. ZAP70 is also required for TCR-CD247/CD3Z internalization and degradation through interaction with the E3 ubiquitin-protein ligase CBL and adapter proteins SLA and SLA2. Thus, ZAP70 regulates both T-cell activation switch on and switch off by modulating TCR expression at the T-cell surface. During thymocyte development, ZAP70 promotes survival and cell-cycle progression of developing thymocytes before positive selection (when cells are still CD4/CD8 double negative). Additionally, ZAP70-dependent signaling pathway may also contribute to primary B-cells formation and activation through B-cell receptor (BCR).
Cellular Location: Cytoplasm. Cell membrane; Peripheral membrane protein. Note: In quiescent T-lymphocytes, it is cytoplasmic. Upon TCR activation, it is recruited at the plasma membrane by interacting with CD247/CD3Z. Colocalizes together with RHOH in the immunological synapse. RHOH is required for its proper localization to the cell membrane and cytoskeleton fractions in the thymocytes (By similarity). Tissue Location: Expressed in T- and natural killer cells. Also present in early thymocytes and pro/pre B-cells. The following patways have been known to be associated with this gene. Abnormalities, Multiple, Cell Transformation, Neoplastic, Immunologic Deficiency Syndromes, Inflammation, Kidney Diseases, Necrosis, Neoplasms, Experimental, Neutropenia, Severe Combined Immunodeficiency, Vascular Diseases are some of the diseases may be linked to ZAP70 Antibody (Center) Blocking Peptide. ZAP70 also interacts with the following gene(s): CBL, CD247, CD3E, LAT, LCK, LCP2, PLCG1, SHC1, VAV1. Blood, Bone Marrow, Brain, Intestine, Kidney, Liver, Lymph Node, Prostate, Spleen, Thymus tissues are correlated with this protein.