|The Human WNT4 wnt4 (Catalog #MBS934091) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS934091 ELISA Kit recognizes Human (General) WNT4.
The WNT4 wnt4 product has the following accession number(s) (GI #17402922) (NCBI Accession #NP_110388.2) (Uniprot Accession #P56705). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
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Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Human Protein Wnt-4, WNT4 ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for WNT4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any WNT4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for WNT4 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of WNT4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Samples: Serum, plasma, tissue homogenates
Assay Type: Sandwich
Detection Range: 23.5 pg/ml -1500 pg/ml.
Sensitivity: The minimum detectable dose of human WNT4 is typically less than 5.8 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess. Human Protein Wnt-4, WNT4 ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit. Breast Neoplasms, Disease Models, Animal, Disorders of Sex Development, Female Urogenital Diseases, Fibrosis, Kidney Diseases, Liver Diseases, Neoplasms, Rokitansky Kuster Hauser syndrome, Urogenital Abnormalities are some of the diseases may be linked to Human Protein Wnt-4, WNT4 ELISA Kit. Brain, Embryonic Tissue, Eye, Heart, Kidney, Lung, Mammary Gland, Ovary, Pancreas, Uterus tissues are correlated with this protein. The following patways have been known to be associated with this gene. WNT4 also interacts with the following gene(s): FZD6, SFRP1, SFRP2.