VB12 elisa kit product blog
Tags: ELISA Kit; Bovine ELISA Kit; VB12 elisa kit; VB12; Vitamin B12, VB12;
The Bovine VB12 n/a (Catalog #MBS702114) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS702114 ELISA Kit recognizes Bovine VB12.To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Bovine Vitamin B12, VB12 ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Principle of the assay: This assay employs the direct competitive inhibition enzyme immunoassay technique. Antibody specific for VB12 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells with biotin-conjugated VB12. A competitive inhibition reaction is launched between VB12 (Standards or samples) and biotin-conjugated VB12 with the pre-coated VB12 antibody. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in opposite to the amount of VB12 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Samples: Serum, plasma, tissue homogenates
Assay Type: Competitive
Detection Range: 0.312 pg/ml -20 pg/ml.
Sensitivity: The minimum detectable dose of bovine VB12 is typically less than 0.078 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest bovine VB12 concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess. Bovine Vitamin B12, VB12 ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.