|The Van91I n/a (Catalog #MBS638394) is a Restriction Enzyme and is intended for research purposes only. The product is available for immediate purchase.
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5\'-C C A N N N N^N T G G-3\'.
Dilution Buffer: 10mM Tris-HCl (pH 7.4 at 25 degree C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol.
Digestion of Agarose-embedded DNA: A minimum of 5 units of enzyme is required for digestion of 1ug of agarose-embedded lambda DNA in 16 hours.
Methylation Effects: Van91I does not cut Cm5CA(N)5TGG. Blocked by overlapping Dcm methylation.
Blue/White Cloning Assay: The mix of pUC57/HindIII, pUC57/Eco32I and pUC57/PstI digests was incubated with 10 units of enzyme for 16 hours. After religation and transformation 0.2% of white colonies were detected. Labeled Oligonucleotide (LO) Assay: No detectable degradation of a single-stranded and double-stranded labeled oligonucleotide was observed after incubation with 10 units of restriction endonuclease for 4 hours.
Ligation/Recutting Assay: After 50-fold overdigestion (3u/ug DNA x 17 hours) with Van91I, more than 90% of the DNA fragments can be ligated at a 5\'-termini concentration of 0.1uM. More than 90% of these can be recut.
Overdigestion Assay: No detectable change in the specific fragmentation pattern is observed after 160-fold overdigestion (10u/ug lambda DNA x 16 hours) with Van91I.
Stability During Prolonged Incubation: A minimum of 0.1 units of enzyme is required for complete digestion of 1ug of lambda DNA in 16 hours at 37 degree C.
Storage Buffer: 10mM Tris-HCl (pH 7.4 at 25 degree C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA and 50% glycerol.
Thermal Inactivation: Enzyme is inactivated by incubation at 65 degree C for 20min.
Unit Definition: One unit is defined as the amount of enzyme required to digest 1ug of lambda DNA in 1 hour at 37 degree C in 50ul of assay buffer.