Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource  rightarrow Blog  rightarrow Van91I restriction enzyme  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Blogs moreseparator
separator
 • anti-HNE antibody blog
 • LY294002 inhibitor blog
 • IL-10 elisa set blog
 • Homarus americanus lectin blog
 • NTRK2 elisa kit blog
 • APPL1 elisa kit blog
 • Tissue Set, BioAssay, DNA, RNA, Protein, Monkey tissue set blog
 • QUANTUM FITC-5 MESF accessory reagent blog
 • CRP elisa kit blog
 • DCLK2 sirna blog
 • PGE2 elisa kit blog
 • ZNF71 blocking peptide blog
 • anti-NCK2 antibody blog
 • anti-CALCA antibody blog
 • Amuvatinib inhibitor blog
 • Levanase,partial recombinant protein blog
 • IgG secondary antibody blog
 • anti-dam antibody blog
 • IMA elisa kit blog
 • PDK4 blocking peptide blog
separator
Blog Categories separator
separator
 • Assay Kit Blogs
 • Biochemical Blogs
 • cDNA Clone Blogs
 • Monoclonal Antibody Blogs
 • Polyclonal Antibody Blogs
 • Rec/Purified Protein Blogs
 • Popular Product Blogs
separator
Our Best Sellers moreseparator
separator
 • SIX1 Antibody
 • FER (Phospho-Tyr402) Antibody
 • Cytochrome P450 2A7 (CYP2A7) Antibody
 • GPS2 Antibody
 • Hepatocyte Nuclear Factor 1 Beta (HNF1beta) ELISA Kit
 • Tissue Factor Pathway Inhibitor 2 (TFPI2) ELISA Kit
 • Pulmonary Surfactant Associated Protein B (SPB) ELISA Kit
 • Chondroitin Sulfate (CS) ELISA Kit
 • Cyclin D2 (CCND2) ELISA Kit
 • Bcl2 Associated Death Promoter (BAD) ELISA Kit
 • Toll Like Receptor4 (TLR4) ELISA Kit
 • Atrial Natriuretic Peptide (ANP) ELISA Kit
 • Complement Component 5 (C5) ELISA Kit
 • Vascular Endothelial Cell Growth Factor B (VEGF-B) ELISA Kit
 downarrow more ...
separator

Van91I restriction enzyme product blog

separator
Posted on 2016-01-31 06:49:38 by mybiosource_staff
Share it on ...     
separator
separator
Tags: Restriction Enzyme; Van91I; Van91I restriction enzyme;
separator
The Van91I n/a (Catalog #MBS638394) is a Restriction Enzyme and is intended for research purposes only. The product is available for immediate purchase.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


5\'-C C A N N N N^N T G G-3\'.

Van91I n/a

Dilution Buffer: 10mM Tris-HCl (pH 7.4 at 25 degree C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol.
Digestion of Agarose-embedded DNA: A minimum of 5 units of enzyme is required for digestion of 1ug of agarose-embedded lambda DNA in 16 hours.
Methylation Effects: Van91I does not cut Cm5CA(N)5TGG. Blocked by overlapping Dcm methylation.
pACYC177: 1
pACYC184: 4
Blue/White Cloning Assay: The mix of pUC57/HindIII, pUC57/Eco32I and pUC57/PstI digests was incubated with 10 units of enzyme for 16 hours. After religation and transformation 0.2% of white colonies were detected. Labeled Oligonucleotide (LO) Assay: No detectable degradation of a single-stranded and double-stranded labeled oligonucleotide was observed after incubation with 10 units of restriction endonuclease for 4 hours.
Lambda: 14
Ligation/Recutting Assay: After 50-fold overdigestion (3u/ug DNA x 17 hours) with Van91I, more than 90% of the DNA fragments can be ligated at a 5\'-termini concentration of 0.1uM. More than 90% of these can be recut.
M13mp18/19: 0
Overdigestion Assay: No detectable change in the specific fragmentation pattern is observed after 160-fold overdigestion (10u/ug lambda DNA x 16 hours) with Van91I.
pBR322: 2
PhiX174: 2
pTZ19R/U: 0
pUC18/19: 0
pUC57: 0
Stability During Prolonged Incubation: A minimum of 0.1 units of enzyme is required for complete digestion of 1ug of lambda DNA in 16 hours at 37 degree C.
Storage Buffer: 10mM Tris-HCl (pH 7.4 at 25 degree C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA and 50% glycerol.

Thermal Inactivation: Enzyme is inactivated by incubation at 65 degree C for 20min.
Unit Definition: One unit is defined as the amount of enzyme required to digest 1ug of lambda DNA in 1 hour at 37 degree C in 50ul of assay buffer.
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions