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Van91I restriction enzyme product blog

Posted on 2016-01-31 06:49:38 by mybiosource_staff
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Tags: Restriction Enzyme; Van91I; Van91I restriction enzyme;
The Van91I n/a (Catalog #MBS638394) is a Restriction Enzyme and is intended for research purposes only. The product is available for immediate purchase.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

5\'-C C A N N N N^N T G G-3\'.

Van91I n/a

Dilution Buffer: 10mM Tris-HCl (pH 7.4 at 25 degree C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol.
Digestion of Agarose-embedded DNA: A minimum of 5 units of enzyme is required for digestion of 1ug of agarose-embedded lambda DNA in 16 hours.
Methylation Effects: Van91I does not cut Cm5CA(N)5TGG. Blocked by overlapping Dcm methylation.
pACYC177: 1
pACYC184: 4
Blue/White Cloning Assay: The mix of pUC57/HindIII, pUC57/Eco32I and pUC57/PstI digests was incubated with 10 units of enzyme for 16 hours. After religation and transformation 0.2% of white colonies were detected. Labeled Oligonucleotide (LO) Assay: No detectable degradation of a single-stranded and double-stranded labeled oligonucleotide was observed after incubation with 10 units of restriction endonuclease for 4 hours.
Lambda: 14
Ligation/Recutting Assay: After 50-fold overdigestion (3u/ug DNA x 17 hours) with Van91I, more than 90% of the DNA fragments can be ligated at a 5\'-termini concentration of 0.1uM. More than 90% of these can be recut.
M13mp18/19: 0
Overdigestion Assay: No detectable change in the specific fragmentation pattern is observed after 160-fold overdigestion (10u/ug lambda DNA x 16 hours) with Van91I.
pBR322: 2
PhiX174: 2
pTZ19R/U: 0
pUC18/19: 0
pUC57: 0
Stability During Prolonged Incubation: A minimum of 0.1 units of enzyme is required for complete digestion of 1ug of lambda DNA in 16 hours at 37 degree C.
Storage Buffer: 10mM Tris-HCl (pH 7.4 at 25 degree C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA and 50% glycerol.

Thermal Inactivation: Enzyme is inactivated by incubation at 65 degree C for 20min.
Unit Definition: One unit is defined as the amount of enzyme required to digest 1ug of lambda DNA in 1 hour at 37 degree C in 50ul of assay buffer.
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