VA elisa kit product blog
Tags: ELISA Kit; Horse ELISA Kit; VA elisa kit; Vitamin A (VA);
The Horse VA n/a (Catalog #MBS705949) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS705949 ELISA Kit recognizes Horse VA.To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to VA. Standards or samples are added to the appropriate microtiter plate wells with Horseradish Peroxidase (HRP) conjugated VA. The competitive inhibition reaction is launched between with HRP-conjugated VA and VA in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of VA in the sample. The color development is stopped and the intensity of the color is measured.
Samples: Serum, plasma.
Assay Type: Competitive
Detection Range: 1.875 ng/ml -30 ng/ml.
Sensitivity: 1.402 ng/ml. Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision: Inter-assay Precision (Precision between assays):CV%<10%. Three samples of known concentration were tested in twenty assays to assess. Horse Vitamin A, VA ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.