Universal Tissue cDNA, Reverse Transcribed by Oligo dT Primer, Chicken Adult Normal, BioGenomics cdna product blog
Tags: cDNA; Universal Tissue cDNA, Reverse Transcribed by Oligo dT Primer, Chicken Adult Normal, BioGenomics;
The Universal Tissue cDNA, Reverse Transcribed by Oligo dT Primer, Chicken Adult Normal, BioGenomics n/a (Catalog #MBS652169) is a cDNA Clone and is intended for research purposes only. The product is available for immediate purchase.Suitable for use in SNP analysis, Southern Blot, PCR, Genomic DNA library construction, Profiling study in gene expression. Researchers should empirically determine the suitability of the Universal Tissue cDNA, Reverse Transcribed by Oligo dT Primer, Chicken Adult Normal, BioGenomics n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Bioselect Tissue cDNA: cDNA is supplied as First Strand, Multiple Tissue Panels, and Matched Pairs. PCR-ready First Strand cDNA is tissue specific and are ready-to-use for gene discovery or expression analysis. Over 350 cDNAs from human adult and fetal normal tissues, human diseased and tumor tissues, rat, mouse, monkey and plant tissues are included in this extensive collection.
BioSelect Universal cDNAMixed Male and Female donors. Prepared by reverse transcribed RNA by using random hexamer or oligo dT primer. Total RNA is isolated by modified guanidine thiocyanate techniques. The Universal cDNA serves as a standard for comparison of gene expression by real time PCR and regular PCR, and also as a gene pool for cloning genes.
Supplied With: T5595-0010: Tissue, cDNA, Actin Control Primer. Quality Control: RNA Integrity for cDNA synthesis examined by visual inspection for the presence of intact bands of 18s and 28s ribosomal RNA using denaturing agarose gel.
Quality and purity of total RNA is determined spectrophotometrically.
A260/280 1.8 - 2.0 (10mM Tris-Cl, pH 7.5)
8S/18S Ratio 1
•. RNA used for cDNA synthesis is treated by DNase I and is tested as DNA-free RNA by PCR.
• The synthesized human, animal, and cell line cDNA was 5\' selected to ensure its full length.
• The cDNA was used as template for PCR amplification of beta-actin gene and an 838 bp beta-actin band was visualized on 1% agarose gel
• Synthesized plant cDNA was used as template for PCR amplification of chloroplast gene.
• 458bp chloroplast band visualized on 1% agarose gel.
-actin control primer included, 10 PCR reactions
Chloroplast control primer included., 10 PCR reactions.