Trypsin, Bovine Pancreas, TPCK Treated enzyme product blog
Tags: Enzyme; Trypsin, Bovine Pancreas, TPCK Treated; Trypsin, Bovine Pancreas, TPCK Treated enzyme;
The Trypsin, Bovine Pancreas, TPCK Treated n/a (Catalog #MBS653896) is an Enzyme produced from Bovine pancreas and is intended for research purposes only. The product is available for immediate purchase.The Trypsin, Bovine Pancreas, TPCK Treated n/a product has the following accession number(s) (GI #156867562) (NCBI Accession #EDO60934.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
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Trypsin is a pancreatic serine protease with substrate specificity based upon positively charged lysine and arginine side chains (Brown and Wold, 1973a). It has been isolated from a number of sources including dogfish (Titani, et al., 1975), moose (Stevenson and Voordouw, 1975), whale (Bricteux-Gregoire, et al., 1975), elephant seal (Bricteux-Gegoire, et al., 1974), Streptomyces griseus (Jurasek and Smillie, 1974; Olafson and Smillie, 1975), canine (Ohlsson and Tegner, 1973), African lungfish (Reeck and Neurath, 1972), swine (Hermodson, et al., 1973; Walker and Keil, 1973), rat (Vandermeers, et al., 1973), human (Mallory and Travis, 1973; Figarell, et al., 1975). Trypsinogen and trypsin have been reviewed by Walsh (1970). The enzyme exhibits esterase and amidase activities.
CAS Number: 9002-07-7
Extinction Coefficient: = 14.3.
Isoelectric Point: Trypsinogen: pH 9.3 (Walsh and Neurath, 1964).
Trypsin: pH 10.5 (Cunningham, 1954). Quality Control: SDS-PAGE
Unit Definition: 1 unit hydrolyzes 1 umole of p-toluene-sulfonyl-L-arginine methyl ester (TAME) per minute at 25ºC, pH 8.2, in the presence of 0.01M calcium ion. (1 TAME unit = 19.2 USP/NF units = 57.5 BAEE units).