|The Human TNF-alpha tnf (Catalog #MBS914766) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS914766 ELISA Kit recognizes Human (General) TNF-alpha.
The TNF-alpha tnf product has the following accession number(s) (GI #37210) (NCBI Accession #CAA26669.1) (Uniprot Accession #P01375). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
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Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Human Tumor necrosis factor alpha, TNF-alpha antibody ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This assay employs the qualitative enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with antigen. Samples are pipetted into the wells with anti-human immunoglobulin conjugated Horseradish Peroxidase (HRP). Any antibodies specific for the antigen present will bind to the pre-coated antigen. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of human tumor necrosis factor alpha (TNF-alpha) antibody bound in the initial step. The color development is stopped and the intensity of the color is measured.
Samples: Serum, plasma. Detection Wavelength: 450nm
Sample Volume: 50-100ul. Human Tumor necrosis factor alpha, TNF-alpha antibody ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit. Blood, Brain, Connective Tissue, Heart, Intestine, Lung, Lymph Node, Ovary, Placenta, Prostate tissues are correlated with this protein. Hypersensitivity, Infection, Inflammation, Inflammatory Bowel Diseases, Liver Diseases, Lung Diseases, Necrosis, Sepsis, Skin Diseases, Vascular Diseases are some of the diseases may be linked to Human Tumor necrosis factor alpha, TNF-alpha antibody ELISA Kit. The following patways have been known to be associated with this gene. TNF-alpha also interacts with the following gene(s): ADAM17, ADAM9, BGN, CSF1, DCN, IFNG, PRTN3, TNFRSF1A, TNFRSF1B, TRAF2.