TMB accessory reagent product blog
Tags: Accessory Reagent; TMB CORE+; TMB accessory reagent; TMB;
The TMB n/a (Catalog #MBS239112) is an Accessory Reagent and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s TMB CORE+ can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA).ELISA: Minimum Dilution: Neat. Researchers should empirically determine the suitability of the TMB n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Intended Use: This item is a high performance TMB (3,3�, 5, 5�- tetramethylbenzidine) solution, recommended for use in ELISA as a substrate for horseradish peroxidase (HRP).
This item contains TMB, substrate buffer and hydrogen peroxide in a safe, ready to use solution. The activity of TMB has been optimised to enable increased sensitivity, minimal background and rapid development.
This item produces a deep blue colour during the enzymatic degradation of H2O2 by horseradish Peroxidase. The reaction may be stopped with 0.2M sulphuric acid, resulting in a yellow colour read at 450nm.
Instructions For Use: 1. It is recommended that 100ul of this item TMB substrate is used per microtiter well. Pour the desired amount of substrate into a sealed container and allow it to reach room termperature in the dark.
2. Add 100ul substrate solution per microtiter well.
3. Allow development of the substrate solution. Time of development is typically 5-30 minutes. For best results, the plate should be kept in the dark during incubation e.g. wrapped in tinfoil.
4. For kinetic assays, read absorbance at 655nm (blue). For endpoint assays, add an equal volume of 0.2M sulphuric acid and read the absorbance at 450nm (yellow). This endpoint solution is stable for up to one hour.
N.B. If reduced intensity is required, it is recommended that the development time is reduced or the antibody/conjugate is diluted further. (Dilution of This item is not recommended).