Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource  rightarrow Blog  rightarrow TLR5 elisa kit  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Blogs moreseparator
separator
 • IgG secondary antibody blog
 • Lercanidipine inhibitor blog
 • AMH recombinant protein blog
 • MTMR12 sirna blog
 • Kynurenine elisa kit blog
 • USP40 sirna blog
 • Tissue, Section Disease, Alzheimer's Disease, Brain, Postcentral Gyrus tissue section blog
 • ACADS recombinant protein blog
 • Penk peptide blog
 • SNX26 peptide blog
 • anti-MB21D1 antibody blog
 • PEBP1 inhibitor blog
 • Valproic acid (BSA) conjugate antigen blog
 • Rubeola, Strain Edmonston infectious disease blog
 • ADAMTS4 sirna blog
 • anti-CST3 antibody blog
 • Bsu15I restriction enzyme blog
 • CBX7 peptide blog
 • SUV39H2 sirna blog
 • NCF1 blocking peptide blog
separator
Blog Categories separator
separator
 • Assay Kit Blogs
 • Biochemical Blogs
 • cDNA Clone Blogs
 • Monoclonal Antibody Blogs
 • Polyclonal Antibody Blogs
 • Rec/Purified Protein Blogs
 • Popular Product Blogs
separator
Our Best Sellers moreseparator
separator
 • Polymyxin B Antibody
 • GSK3 beta (GSK3B) Antibody
 • PECAM1 Antibody
 • P 38 Mitogen-Activated Protein Kinase (P38MAPK) ELISA Kit
 • AKR1B10 Antibody
 • Delta homolog 2 (Dlk2) Recombinant Protein
 • A disintegrin and metalloproteinase with thrombospondin moti... ELISA Kit
 • Abeta42 (Amyloid Beta 42) (Ab42) CLIA Kit
 • ATF2 Antibody
 • IL-5 (IL5) ELISA Kit
 • Salmonella species Antibody
 • Serum paraoxonase-arylesterase 2 (PON2) ELISA Kit
 • MDC Recombinant Protein
 • Ovalbumin specific Immunoglobulin G1 (OVA sIgG1) ELISA Kit
 downarrow more ...
separator

TLR5 elisa kit product blog

separator
Posted on 2014-06-03 08:47:44 by mybiosource_staff
Share it on ...     
separator
separator
Tags: ELISA Kit; Human ELISA Kit; TLR5; toll-like receptor 5; TLR5 elisa kit;
separator
The Human TLR5 tlr5 (Catalog #MBS700493) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS700493 ELISA Kit recognizes Human (General) TLR5.

The TLR5 tlr5 product has the following accession number(s) (GI #16751843) (NCBI Accession #NP_003259.2) (Uniprot Accession #O60602). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


(Or you can also download the PDF Manual for complete product instructions).

Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Human Toll-like receptor 5, TLR-5 ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only) TLR5.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to TLR-5. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for TLR-5 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3\',5,5\' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain TLR-5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of TLR-5 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Detection Range: 1.25 ng/ml-80 ng/ml
Sensitivity: 0.32 ng/ml. Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Detection Wavelength: 450 nm
Sample Volume: 50-100ul
Protein Biological Process 1: Immunity
Protein Biological Process 3: Immunity. Human Toll-like receptor 5, TLR-5 ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit. The following patways have been known to be associated with this gene. Brain Diseases, Heart Diseases, Hepatitis, Inflammation, Kidney Diseases, Legionnaires\' Disease, Liver Diseases, Lupus Erythematosus, Systemic, Necrosis, Weight Loss are some of the diseases may be linked to Human Toll-like receptor 5, TLR-5 ELISA Kit. Brain, Connective Tissue, Kidney, Liver, Lung, Mouth, Ovary, Skin, Testis, Uterus tissues are correlated with this protein.
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions