Tissue Set, BioAssay, DNA, RNA, Protein Adult Normal, Adipose tissue array product blog
Tags: Tissue Array; Tissue Set, BioAssay, DNA, RNA, Protein Adult Normal, Adipose; Tissue Set, BioAssay, DNA, RNA, Protein Adult Normal, Adipose tissue array;
The Tissue Set, BioAssay, DNA, RNA, Protein Adult Normal, Adipose n/a (Catalog #MBS639651) is a Tissue Array and is intended for research purposes only. The product is available for immediate purchase.To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
BioAssay Tissue Set includes Genomic DNA, RNA, and Protein isolated from the same piece of biomaterials, such as tissues or cells. Set includes 50ug Total RNA 10ug Genomic DNA, and 100ug Protein. Set is particularly important for functional genomics and proteomics study, such as gene regulation, expression of genes and protein, among DNA, RNA, and protein.
Produced by proprietary techniques, this set exhibits the same high qualities as those products isolated separately by conventional methods. Compared to commercial kits and reagents for RNA isolation, which can produce varying quality of genomic DNA and protein, the RNA quality is typically inferior due to protein isolation methods, which can yield only 10-30%. This means many proteins may be missing, and is not suitable for protein expression profiling, protein quantitation and protein purification.
In contrast, the BioAssay Tissue Set is ready to use and contains highly purified DNA, RNA and Protein suitable for use in protein expression profiling, protein quantitation, and protein purification.
Total Protein: 1. The isolated protein pattern on SDS-PAGE gel is visualized by coomassie blue staining, the pattern is consistent with each lot.
2. The isolated protein is Western analyzed by either GAPDH or b-actin antibody, and the expression level is consistent with each lot.
Note: Due to the nature of tissues, some time even house keeping genes show weak signal or no signal in some tissues. So GAPDH and b-actin cannot be guaranteed to show strong signals at the same time. Human Adult Normal Tissue: Uterus: Cervix
Quality Control: Genomic DNA:
1. The quality and purity of genomic DNA were tested by spectrophotometer and electrophoresis. A260/280 is between 1.8 and 2.0 (detected in 10mM Tris-Cl, pH 7.5).
2. RNase treatment to ensure the RNA contamination is eliminated.
3. Hind III digestion test: genomic DNA was successfully digested by Hind III.
4. PCR test: A specific fragment of actin gene, about 800bp, was successfully amplified from the genomic DNA.