|The Tissue cDNA, First Strand Diseased n/a (Catalog #MBS652445) is a cDNA and is intended for research purposes only. The product is available for immediate purchase.
Immediate PCR Amplification of known genes, Verification of genetic mutation, Comparison of a specific gene between different tissues, Analysis of mRNA alternative splicing, Gene cloning and target sequencing. Researchers should empirically determine the suitability of the Tissue cDNA, First Strand Diseased n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
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Bioselect Tissue cDNA: cDNA is supplied as First Strand, Multiple Tissue Panels, and Matched Pairs. PCR-ready First Strand cDNA is tissue specific and are ready-to-use for gene discovery or expression analysis. Over 350 cDNAs from human adult and fetal normal tissues, human diseased and tumor tissues, rat, mouse, monkey and plant tissues are included in this extensive collection.
PCR Ready First Strand cDNAs is an excellent source of tissue specific, PCR-ready cDNA, and it can be immediately used for gene discovery or expression analysis. First-Strand cDNA is synthesized from RNA isolated from a wide variety of documented human adult and fetal normal tissues, human diseased and tumor tissues, mouse, rat, monkey, plant and other tissues. Total RNA used for cDNA synthesis is isolated by modified guanidine thiocyanate techniques. 11ug total RNA was primed by an oligo dT primer and reverse transcribed by MMLV reverse transcriptase in 40ul final volume. RT Reaction stopped by heating at 65 degree C for 10 minutes.
1ul cDNA is good for one PCR reaction. The 5\' end of human clathrin cDNA (a 6kb gene) has been amplified by PCR from all of the cDNAs. Features: Ready to use for PCR
Oligo dT primer used to ensure the entire 3\' end of cDNA is present
With some cDNA used as templates, 12kb PCR amplicon was obtained to ensure the intactness of cDNA
The largest selection of cDNAs from different tissues on the market
Documentation of tissues\' clinical histories available (additional cost)
Quality Control: 1. The integrity of the RNA used for cDNA synthesis is examined by visual inspection for the presence of intact bands of 18s and 28s ribosomal RNA when electrophoresed on a denaturing agarose gel. The quality and purity of total RNA were tested by spectrophotometer. A260/280 is between 1.8 and 2.0 (detected in 10mM Tris-Cl, pH 7.5). The ratio of 28S/18S is 1.
2. The RNA used for cDNA synthesis is treated by DNase I, and is tested as DNA free RNA by PCR.
3. The synthesized human, animal, and cell line cDNA was 5\' selected to ensure its full length. The cDNA was used as template for PCR amplification of -actin gene and an 838 bp -actin band was visualized on 1% agarose gel. -actin control primer is included. It is enough for 10 PCR reactions.
4. The synthesized plant cDNA was used as template for PCR amplification of chloroplast gene. A 458 bp chloroplast band was visualized on 1% agarose gel. Chloroplast control primer is included. It is enough for 10 PCR reactions.