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Tissue cDNA, First Strand Adult Normal, Tonsil, BioGenomics cdna product blog

Posted on 2016-02-29 15:42:55 by mybiosource_staff
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Tags: cDNA; Tissue cDNA, First Strand Adult Normal, Tonsil, BioGenomics; Tissue cDNA, First Strand Adult Normal, Tonsil, BioGenomics cdna;
The Tissue cDNA, First Strand Adult Normal, Tonsil, BioGenomics n/a (Catalog #MBS652456) is a cDNA and is intended for research purposes only. The product is available for immediate purchase.
Immediate PCR Amplification of known genes, Verification of genetic mutation, Comparison of a specific gene between different tissues, Analysis of mRNA alternative splicing, Gene cloning and target sequencing. Researchers should empirically determine the suitability of the Tissue cDNA, First Strand Adult Normal, Tonsil, BioGenomics n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Tissue cDNA, First Strand Adult Normal, Tonsil, BioGenomics n/a

Bioselect Tissue cDNA: cDNA is supplied as First Strand, Multiple Tissue Panels, and Matched Pairs. PCR-ready First Strand cDNA is tissue specific and are ready-to-use for gene discovery or expression analysis. Over 350 cDNAs from human adult and fetal normal tissues, human diseased and tumor tissues, rat, mouse, monkey and plant tissues are included in this extensive collection.
Quality Control: 1. The integrity of the RNA used for cDNA synthesis is examined by visual inspection for the presence of intact bands of 18s and 28s ribosomal RNA when electrophoresed on a denaturing agarose gel. The quality and purity of total RNA were tested by spectrophotometer. A260/280 is between 1.8 and 2.0 (detected in 10mM Tris-Cl, pH7.5). The ratio of 28s/18s is >= 1.
2. The RNA used for cDNA synthesis is treated by DNase I, and is tested as DNA free RNA by PCR.
3. The synthesized human, animal, and cell line cSDNA was 5 selected to ensure its full length. The cDNA was used as template for PCR amplification of beta-actin gene and an 838 bp beta-actin band was visualized on 1% agarose gel. Beta-actin control primer is included.

b-Acting Control primer (included).
Unit: 1ul cDNA is good for on PCR reaction.
Donor Information: DNA from 54 years old, normal, male, human tonsil.
Control PCR Condition: Ready First Strand cDNA: 1.0ul
10 X PCR Buffer: 2.5ul
10mM dNTP: 0.5ul
Control Primers (5uM): 1.0ul
H2O, Neclease-free: 19.8ul
Tag Polymerase (5u/ul): 0.2ul
PCR Thermocycling: 94 degree C x 2 minutes, 1 cycle.
94 degre C x 30 seconds, 55 degree C x 30 seconds, 72 degree C x 30 seconds, 35 cycles.
72 degree C x 5 minutes, 1 cycle. Then hold at 4 degree C. Features: Ready to use for PCR
Oligo dT primer used to ensure the entire 3\' end of cDNA is present
With some cDNA used as templates, 12kb PCR amplicon was obtained to ensure the intactness of cDNA
The largest selection of cDNAs from different tissues on the market
Documentation of tissues\' clinical histories available (additional cost)
Uniques Features of cDNA: PCR Ready First Strand cDNA is made from our high-quality total RNA. Each cDNA is a premade, tissue-specific "pool" of first strand cDNA from which full-length genes can be amplified by using sets of gene-specific primers. They can also be used to study tissue-specific gene expression and to find not only polymorphic forms of mRNA but also mRNA belonging to a multigene family. The following features have been integrated into our cDNA products to ensure their superior quality.
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