Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
Fax 1.858.633.0166    Email 
menu1 Home Products Ordering Company Help menu7
   MyBioSource  rightarrow Blog  rightarrow THP-1 lysate  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Blogs moreseparator
 • CALCA peptide blog
 • ZBTB11 sirna blog
 • KNCN sirna blog
 • IGF-1 elisa kit blog
 • anti-IRF3 antibody blog
 • hsa-miR-3187-5p mirna blog
 • AVPR1B blocking peptide blog
 • SOD3 recombinant protein blog
 • IgG secondary antibody blog
 • C22orf46 sirna blog
 • anti-TG antibody blog
 • FCAR blocking peptide blog
 • SOCS4 recombinant protein blog
 • PDE7A blocking peptide blog
 • hsa-miR-6752-3p mirna blog
 • Liver lysate blog
 • ALPI elisa kit blog
 • RBMY1B sirna blog
 • GCLC blocking peptide blog
 • anti-RPLP0 antibody blog
Blog Categories separator
 • Assay Kit Blogs
 • Biochemical Blogs
 • cDNA Clone Blogs
 • Monoclonal Antibody Blogs
 • Polyclonal Antibody Blogs
 • Rec/Purified Protein Blogs
 • Popular Product Blogs
Our Best Sellers moreseparator
 • IMPA1 Recombinant Protein
 • CEP83 Antibody
 • S-Adenosylmethionine (SAM) and S-Adenosylhomocysteine (SAH) ELISA Kit
 • Tumor Necrosis Factor alpha (TNFalpha) ELISA Kit
 • PLD1 Antibody
 • CytoSelect BrdU Cell Proliferation ELISA Kit
 • HSD17B13 Antibody
 • HSV 1-2 IgG ELISA Kit
 • Alpha-synuclein (SNCA) Antibody
 • Alpha-Tocopherol (vitamine E) (BSA) conjugate Antigen
 • BFP Antibody
 • GFP Antibody
 • Protein Kinase C Delta (PKCd) ELISA Kit
 downarrow more ...

THP-1 lysate product blog

Posted on 2015-09-09 02:30:23 by mybiosource_staff
Share it on ...     
Tags: Lysate; THP-1; THP-1 lysate;
The THP-1 n/a (Catalog #MBS151681) is a Lysate and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s THP-1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the THP-1 n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

THP-1 lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 mug/ml of aprotinin, 5 mug/ml of leupeptin. Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The THP-1 lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

THP-1 n/a

Species: Human
Buffer: THP-1 lysate is supplied in SDS sample buffer containing 5% Beta-mercaptoethanol.
By Type: Cell Line. Tissue/Cell Type: THP-1 (Human monocyte)
Tissue by Dagnosis: Normal
Diagnosis: Normal
Tissue by Species: Human.
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions