|The Human TGFB2 tgfb2 (Catalog #MBS700392) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS700392 ELISA Kit recognizes Human (General) TGFB2.
The TGFB2 tgfb2 product has the following accession number(s) (GI #208022653) (NCBI Accession #NP_001129071.1) (Uniprot Accession #P61812). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
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Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Human transforming growth factors beta2, TGFbeta2 ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with anantibody specific to TGF-beta2. Standards or samples are then added tothe appropriate microtiter plate wells with a biotin-conjugatedantibody preparation specific for TGF-beta2 and Avidin conjugated toHorseradish Peroxidase (HRP) is added to each microplate well andincubated. Then a TMB (3,3\',5,5\' tetramethyl-benzidine) substratesolution is added to each well. Only those wells that contain TGF-beta2,biotin-conjugated antibody and enzyme-conjugated Avidin will exhibita change in color. The enzyme-substrate reaction is terminated bythe addition of a sulphuric acid solution and the color change ismeasured spectrophotometrically at a wavelength of 450 nm +/- 2 nm.The concentration of TGF-beta2 in the samples is then determined bycomparing the O.D. of the samples to the standard curve.
Samples: Serum, plasma and cell culture supernates
Detection Range: 15.6pg/ml-1000 pg/ml
Sensitivity: 3.9 pg/ml. Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Detection Wavelength: 450 nm
Sample Volume: 50-100ul. Human transforming growth factors beta2, TGFbeta2 ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit. Bone, Brain, Connective Tissue, Embryonic Tissue, Eye, Heart, Lung, Muscle, Skin, Vascular tissues are correlated with this protein. Breast Neoplasms, Cardiovascular Diseases, Fibrosis, Glaucoma, Heart Diseases, Inflammation, Kidney Diseases, Liver Diseases, Necrosis, Nervous System Diseases are some of the diseases may be linked to Human transforming growth factors beta2, TGFbeta2 ELISA Kit.