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TGFB1 elisa kit product blog

Posted on 2014-03-15 00:47:05 by mybiosource_staff
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Tags: ELISA Kit; Human ELISA Kit; TGFB1; TGFB1 elisa kit; transforming growth factor, beta 1;
The Human TGFB1 tgfb1 (Catalog #MBS703954) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS703954 ELISA Kit recognizes Human (General) TGFB1.

The TGFB1 tgfb1 product has the following accession number(s) (GI #63025222) (NCBI Accession #NP_000651.3) (Uniprot Accession #P01137). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

(Or you can also download the PDF Manual for complete product instructions).

Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Human Transforming Growth factor beta1, TGF-beta1 ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only) TGFB1.

Introduction: Transforming growth factor beta 1 or TGF-beta1 is a polypeptide member of the transforming growth factor beta superfamily of cytokines. It is a secreted protein hat performs many cellular functions, including the control of cell growth, cell proliferation, cell differentiation and apoptosis. TGF-beta1 was first identified in human platelets as a protein with a molecular mass of 25 kilodaltons with a potential role in wound healing. It was later characterized as a large protein precursor (containing 390 amino acids) that was proteolytically processed to produce a mature peptide of 112 amino acids. TGF-beta1 plays an important role in controlling the immune system, and shows different activities on different types of cell, or cells at different developmental stages. Most immune cells (or leukocytes) secrete TGF-beta1. Some T cells (e.g. regulatory T cells) release TGF-beta1 to inhibit the actions of other T cells. Interleukin 1- and interleukin 2-dependent proliferation of activated T cells and the activation of quiescent helper T cells and cytotoxic T cells is prevented by the activity of TGF-beta1. Similarly, TGF-beta1 can inhibit the secretion and activity of many other cytokines including interferon-gamma, tumor necrosis factor-alpha (TNF-alpha) and various interleukins. It can also decrease the expression levels of cytokine receptors, such as the IL-2 receptor to down-regulate the activity of immune cells. However, TGF-beta1 can also increase the expression of certain cytokines in T cells and promote their proliferation, particularly if the cells are immature. TGF-beta1 has similar effects on B cells that also vary according to the differentiation state of the cell. It inhibits proliferation and apoptosis of B cells, and plays a role in controlling the expression of antibody, transferrin and MHC class II proteins on immature and mature B cells. The effects of TGF-beta1 on macrophages and monocytes are predominantly suppressive; this cytokine can inhibit the proliferation of these cells and prevent their production of reactive oxygen and itrogen intermediates. However, as with other cell types, TGF-beta1 can also have the opposite effect on cells of myeloid origin. For example, TGF-beta1 acts as a hemoattractant, directing an immune response to some pathogens; macrophages and monocytes respond to low levels of TGF-beta1 in a chemotactic manner. urthermore, the expression of monocytic cytokines and phagocytic killing by macrophages can be increased by the action of TGF-beta1.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to TGF-beta1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for TGF-beta1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3\',5,5\' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain TGF-beta1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of TGF-beta1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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