Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource  rightarrow Blog  rightarrow TFR elisa kit  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Blogs moreseparator
separator
 • KCNK15 blocking peptide blog
 • anti-TYMS antibody blog
 • anti-CD164 antibody blog
 • Gabrb2 elisa kit blog
 • ZP2 shrna blog
 • SEMA6D blocking peptide blog
 • Disopyramide inhibitor blog
 • CDHR5 blocking peptide blog
 • anti-CD135 antibody blog
 • ALDH3A1 shrna blog
 • TMEM136 sirna blog
 • TMEM169 shrna blog
 • ALOX12 blocking peptide blog
 • CKM sirna blog
 • anti-Chlamydia trachomatis antibody blog
 • IgG secondary antibody blog
 • anti-GPR98 antibody blog
 • VWF native protein blog
 • anti-PTPRM antibody blog
 • anti-CCT2 antibody blog
separator
Blog Categories separator
separator
 • Assay Kit Blogs
 • Biochemical Blogs
 • cDNA Clone Blogs
 • Monoclonal Antibody Blogs
 • Polyclonal Antibody Blogs
 • Rec/Purified Protein Blogs
 • Popular Product Blogs
separator
Our Best Sellers moreseparator
separator
 • wingless-type MMTV integration site family, member 4 (WNT4) ELISA Kit
 • Toxoplasma IgG ELISA Kit
 • CD180 (TLR4-RP105) (CD180 (TLR4-RP105)) Antibody
 • Interferon beta (IFN-beta) ELISA Kit
 • microalbunminuria (MAU-ALB) ELISA Kit
 • GKRP ELISA Kit
 • Actinin Alpha 3 (ACTN3) ELISA Kit
 • Albumin Antibody
 • Lysine-specific demethylase 3B (KDM3B), partial Recombinant Protein
 • Calprotectin (CP) ELISA Kit
 • HSD17B1 Recombinant Protein
 • Interleukin-2 (IL 2) Recombinant Protein
 • Diamine Oxidase (DAO) ELISA Kit
 • Myelin Transcription Factor 1 (MYT1) Antibody
 downarrow more ...
separator

TFR elisa kit product blog

separator
Posted on 2013-08-14 06:42:54 by mybiosource_staff
Share it on ...     
separator
separator
Tags: ELISA Kit; Sheep ELISA Kit; Transferrin Receptor; TFR elisa kit; TFR;
separator
The Sheep TFR tfrc (Catalog #MBS736215) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS736215 ELISA Kit recognizes Sheep (Ovine, General) TFR.

The TFR tfrc product has the following accession number(s) (GI #339516) (NCBI Accession #AAA61153.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


(Or you can also download the PDF Manual for complete product instructions).

Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Sheep Transferrin Receptor ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only) TFR.

Principle of the Assay: TFR ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-TFR antibody and an TFR-HRP conjugate. The assay sample and buffer are incubated together with TFR-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TFR concentration since TFR from samples and TFR-HRP conjugate compete for the anti-TFR antibody binding site. Since the number of sites is limited, as more sites are occupied by TFR from the sample, fewer sites are left to bind TFR-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TFR concentration in each sample is interpolated from this standard curve.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type: Competitive
Sensitivity: 0.1 ng/mL. Intended Uses: This TFR ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Sheep TFR. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Sheep Transferrin Receptor ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit. Anemia, Anemia, Iron-Deficiency, Brain Diseases, Breast Neoplasms, Colorectal Neoplasms, Disease Models, Animal, Inflammation, Iron Metabolism Disorders, Liver Diseases, Lung Neoplasms are some of the diseases may be linked to Sheep Transferrin Receptor ELISA Kit.
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions