Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
Fax 1.858.633.0166    Email 
menu1 Home Products Ordering Company Help menu7
   MyBioSource  rightarrow Blog  rightarrow TFR elisa kit  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Blogs moreseparator
 • TBC1D28 sirna blog
 • Calcimycin antibiotic blog
 • KATNB1 sirna blog
 • a-Glucosidase, Yeast, a-GluD enzyme blog
 • MATN2 elisa kit blog
 • IgG secondary antibody blog
 • LACTB2 shrna blog
 • KIF18A sirna blog
 • Tumor Tissue Blot II immunoblot blog
 • Piceatannol inhibitor blog
 • A4GALT blocking peptide blog
 • anti-BLVRB antibody blog
 • anti-IL6 antibody blog
 • TWF1 blocking peptide blog
 • PTX3 sirna blog
 • LRRC27 shrna blog
 • PNFKB elisa kit blog
 • anti-CD13 antibody blog
 • phospho-b-Tubulin (Ser-172) Peptide peptide blog
 • GID8 shrna blog
Blog Categories separator
 • Assay Kit Blogs
 • Biochemical Blogs
 • cDNA Clone Blogs
 • Monoclonal Antibody Blogs
 • Polyclonal Antibody Blogs
 • Rec/Purified Protein Blogs
 • Popular Product Blogs
Our Best Sellers moreseparator
 • Angiotensin I Converting Enzyme 2 (ACE2) ELISA Kit
 • G Protein Coupled Estrogen Receptor 1 (GPER) ELISA Kit
 • TIAL1 Recombinant Protein
 • Cancer Antigen 19-9 (CA 19-9) Antigen
 • Alveolar Soft Part Sarcoma Chromosome Region, Candidate 1 (A... Antibody
 • Pentraxin 3, Long (PTX3) ELISA Kit
 • DRAM1 - DRAM Antibody
 • GSTkappa1 ELISA Kit
 • Myosin light polypeptide 6 (MYL6) Antibody
 • cytochrome c, somatic (CYCS) ELISA Kit
 • Melanoma-associated antigen 3 (MAGEA3) Recombinant Protein
 • candida albicans (C.albicans) antibody (IgG) ELISA Kit
 • TMEM173 Antibody
 • NOX4 (NOX 4) ELISA Kit
 downarrow more ...

TFR elisa kit product blog

Posted on 2013-08-14 06:42:54 by mybiosource_staff
Share it on ...     
Tags: ELISA Kit; Sheep ELISA Kit; Transferrin Receptor; TFR elisa kit; TFR;
The Sheep TFR tfrc (Catalog #MBS736215) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS736215 ELISA Kit recognizes Sheep (Ovine, General) TFR.

The TFR tfrc product has the following accession number(s) (GI #339516) (NCBI Accession #AAA61153.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

(Or you can also download the PDF Manual for complete product instructions).

Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Sheep Transferrin Receptor ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only) TFR.

Principle of the Assay: TFR ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-TFR antibody and an TFR-HRP conjugate. The assay sample and buffer are incubated together with TFR-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TFR concentration since TFR from samples and TFR-HRP conjugate compete for the anti-TFR antibody binding site. Since the number of sites is limited, as more sites are occupied by TFR from the sample, fewer sites are left to bind TFR-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TFR concentration in each sample is interpolated from this standard curve.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type: Competitive
Sensitivity: 0.1 ng/mL. Intended Uses: This TFR ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Sheep TFR. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Sheep Transferrin Receptor ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit. Blood, Bone, Brain, Embryonic Tissue, Intestine, Kidney, Liver, Lung, Prostate, Skin tissues are correlated with this protein. TFR also interacts with the following gene(s): B2M, EPO, FTH1, HFE, MYC, STEAP3.
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions