Testosterone lum product blog
Tags: Testosterone; LUM; Testosterone lum;
The Testosterone n/a (Catalog #MBS495145) is a LUM Kit produced from Human and is intended for research purposes only. The product is available for immediate purchase.To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Principle of the assay: Theprincipleofthefollowingchemiluminescenceimmunoassay(LUM)testfollows a two- stepcompetitivebindingscenario.Competitionoccursbetweenanunlabeled antigen(presentinstandards,controlandpatientsamples)andabiotin-labelled ntigen(conjugate)foralimitednumberofantibodybindingsitesonthemicrowell plate.Afterwashingthestreptavidin- horseradishperoxidaseconjugateisincubated andboundtoanyboundbiotinylatedtestosterone.Thewashinganddecanting proceduresremoveunboundmaterials.Afterthesecondwashingstep,the luminescencesubstratesolutionisadded.Therelativeluminescenceunits(RLUs) aremeasuredonamicrotiterplateluminometer.TheRLUvaluesareinversely proportionaltotheconcentrationoftestosteroneinthesample.Asetofcalibrators are usedtoplotastandardcurvefromwhichtheamountoftestosteroneinpatient samplesandcontrolscanbedirectlyread.
Intended Uses: ForthequantitativedeterminationofTestosteroneinhumansalivaby chemiluminescenceimmunoassay(LUM).For invitro useonly. Sensitivity: ThelowerdetectionlimitiscalculatedfromthestandardcurvebydeterminingtheresultingconcentrationofthemeanRLUofCalibratorA(basedon10replicateanalyses)minus2SD.Therefore,thesensitivityoftheIBLTestosteroneSalivaLUMkitis1.0pg/ml.