TCR gamma delta secondary antibody product blog
Tags: Secondary Antibody; TCR gamma delta; TCR gamma delta secondary antibody;
The TCR gamma delta n/a (Catalog #MBS673877) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Anti-Chicken TCRgd-BIOT reacts with Chicken and may cross-react with other species as described in the data sheet. MyBioSource\'s TCR gamma delta can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry; Immunohistochemistry (Acetone Fixed/Frozen Sections); Immunoprecipitation.Research Application(s): In ovo depletion of gamma delta T cells
Flow Cytometry Suggested Dilution: <= 1 ug/10^6 cells
Other Applications: Since application vary, you should determine the optimum working dilution for the product that is appropriate for your specific need. Researchers should empirically determine the suitability of the TCR gamma delta n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Mouse Anti-Chicken TCRgd-BIOT with the following immunoassay(s):
Test Data (Chicken peripheral blood mononuclear cells were stained with Mouse Anti-Chicken TCRYO-PE (5B Cat. No. MBS673878) and Mouse Anti-Chicken CD3-FITC (5B Cat. No. MBS673862).)
The monoclonal antibody TCR-1 precipitates a heterodimer of Mr 90 kDa (two bands of Mr 50 kDa and 40 kDa upon reduction) on chicken peripheral blood T cells. Deglycosylation of the heterodimer yields two polypeptides of Mr 35 kDa and 32 kDa. Unlike human and mouse in which yo cells comprise a minor subset of T lymphocytes in the circulation, the chicken has a relatively large subset of yo T cells. The frequency of TCRgamma/delta cells is typically 20-25% of the total blood T cells but may reach approximately 50% in chickens of six- months of age. The majority of TCRgamma/delta+ cells in the thymus and blood are CD4-CD8- although a small subset of them may express CD8 or CD4 coreceptors. However, when the TCRgamma/delta+ cells migrate into the spleen and intestine most of them begin to express CD8. Although the biological function of yo T cells is unclear, they are clearly capable of cytotoxic activity in vitro. The CD8+ yo T cells may also be involved in down-regulation of the immune response. They cannot, however, induce a graft-vs-host (GVH) reactions.
Immunogen: Outbred chicken thymocytes and Ig-negative blood lymphocytes. Characterization: To ensure lot-to-lot consistency, each batch of monoclonal antibody is tested to conform to characteristics of a standard reference reagent using immunofluorescence staining and analysis on a Becton Dickinson FACScan flow cytometer. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing TCR gamma delta are readily searchable from our website. Different antibodies against the same target such as TCR gamma delta may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.