TCC C5b-9 elisa kit product blog
Tags: ELISA Kit; TCC C5B-9 elisa kit; TCC C5B-9; terminal complement complex C5b-9 (TCC C5b-9); Pig ELISA Kit;
The Pig TCC C5b-9 n/a (Catalog #MBS705563) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS705563 ELISA Kit recognizes Pig TCC C5b-9.To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Pig terminal complement complex C5b-9, TCC C5b-9 ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to IL-22. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for IL-22 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3\',5,5\' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain IL-22, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of IL-22 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Samples: Serum, plasma, tissue homogenates
Detection Range: 18.75 ng/ml-1200 ng/ml
Sensitivity: 4.69 ng/ml. Detection Wavelength: 450 nm
Sample Volume: 50-100ul. Pig terminal complement complex C5b-9, TCC C5b-9 ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.