Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource  rightarrow Blog  rightarrow TBX3 elisa kit  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Blogs moreseparator
separator
 • PDE12 positive control blog
 • anti-PSMD11 antibody blog
 • IL33 blocking peptide blog
 • Goat Anti-Mouse IgG1 (gamma 1 chain specific) secondary antibody blog
 • X-a-D-Glucoside substrate blog
 • anti-CD133 antibody blog
 • FABP7 sirna blog
 • MT-ATP8 recombinant protein blog
 • BDNF blocking peptide blog
 • Tissue, Section, Monkey tissue section blog
 • Vanillyl Alcohol inhibitor blog
 • MPPED1 blocking peptide blog
 • TNF-alpha elisa kit blog
 • Amino-dPEG6-t-butyl reagent blog
 • PALB2 blocking peptide blog
 • SLAIN1 sirna blog
 • Escitalopram Oxalate inhibitor blog
 • SH3D19 sirna blog
 • BRAT1 shrna blog
 • CLDN12 sirna blog
separator
Blog Categories separator
separator
 • Assay Kit Blogs
 • Biochemical Blogs
 • cDNA Clone Blogs
 • Monoclonal Antibody Blogs
 • Polyclonal Antibody Blogs
 • Rec/Purified Protein Blogs
 • Popular Product Blogs
separator
Our Best Sellers moreseparator
separator
 • Thrombospondin-1 (THBS1) Recombinant Protein
 • OGT Antibody
 • lysophosphatidic acid receptor 2 (LPAR2) ELISA Kit
 • AQUAPORIN 8 (Aqp8) Antibody
 • Vascular Endothelial Growth Factor A (VEGFA) ELISA Kit
 • CKLFSF4 ELISA Kit
 • inhibin B (INH-B) ELISA Kit
 • Lysozyme (LYZ) Antibody
 • myomesin family, member 3 (MYOM3) ELISA Kit
 • Histone Deacetylase (HD) ELISA Kit
 • Pyridinoline crosslinks, PYD ELISA Kit
 • Cholera Toxin B subunit Antibody
 • Wall-associated receptor kinase-like 14 (WAKL14) Recombinant Protein
 • peptidylprolyl isomerase B (cyclophilin B) (PPIB) ELISA Kit
 downarrow more ...
separator

TBX3 elisa kit product blog

separator
Posted on 2014-03-14 22:39:12 by mybiosource_staff
Share it on ...     
separator
separator
Tags: ELISA Kit; Human ELISA Kit; TBX3; T Box Protein 3; TBX3 elisa kit;
separator
The Human TBX3 n/a (Catalog #MBS725744) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS725744 ELISA Kit recognizes Human (General) TBX3.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


(Or you can also download the PDF Manual for complete product instructions).

Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Human T Box Protein 3 ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only) TBX3.

Principle of the Assay: TBX3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-TBX3 antibody and an TBX3-HRP conjugate. The assay sample and buffer are incubated together with TBX3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TBX3 concentration since TBX3 from samples and TBX3-HRP conjugate compete for the anti-TBX3 antibody binding site. Since the number of sites is limited, as more sites are occupied by TBX3 from the sample, fewer sites are left to bind TBX3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TBX3 concentration in each sample is interpolated from this standard curve.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type: Competitive
Detection Range: 1.0-25ng/mL
Sensitivity: 0.1ng/mL. Intended Uses: This TBX3 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human TBX3. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Human T Box Protein 3 ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions