Taq DNA Polymerase with 10X Reaction (MgCl2) Buffer reagent product blog
Tags: Reagent; Taq DNA Polymerase with 10X Reaction (MgCl2) Buffer; Taq DNA Polymerase with 10X Reaction (MgCl2) Buffer reagent;
The Taq DNA Polymerase with 10X Reaction (MgCl2) Buffer n/a (Catalog #MBS308022) is a Reagent and is intended for research purposes only. The product is available for immediate purchase.To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given reagent. We\'ve tested the Taq DNA Polymerase with 10X Reaction (MgCl2) Buffer with the following immunoassay(s):
Testing Data(TD)
Testing Data (TD) (Thermal cycling conditions: The following general cycling conditions are recommended but can vary depending on the template and primers being used.)
The Taq DNA Polymerase gene a thermos stable enzyme isolated from Thermus aquaticus YT1 and expressed in E.coli. The enzyme consists of a single polypeptide with a molecular weight of 94 kDa. Taq DNA Polymerase possess a 5� ? 3� polymerase activity, endonuclease activity, and will synthesize DNA products having dA overhangs on the 3� ends.
Taq DNA Polymerase is supplied in a storage buffer containing: 20mM HEPES (pH 7.9), 100mM KCl, 0.1mM EDTA, 1mM DTT, 50% (v/v) Glycerol, and other stabilizers.
100 Units: Number of Tubes:
Taq DNA Polymerase - 100 units (1)
10X Reaction (MgCl2) Buffer - 1ml (1)
Total Volume:
Taq DNA Polymerase - (20uL)
10X Reaction (MgCl2) Buffer - (1mL)
10X Reaction (MgCl2) Buffer: Empirical Taq DNA Polymerase is supplied with a specially optimized reaction buffer containing: 100mM Tris-HCl (pH 8.4), 500mM KCl, 15mM MgCl2. This Buffer is supplied in a 10X concentration and should be diluted for use.