|The Pig T4 n/a (Catalog #MBS704081) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS704081 ELISA Kit recognizes Pig (Porcine, Swine, General) T4.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual
for complete product instructions).
Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Pig Thyroxine, T4 ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. An antibody specific to T4 has been pre-coated onto a microplate. Standards or samples are added to the appropriate microtiter plate wells with biotin-conjugated T4 and incubated. A competitive inhibition reaction is launched between T4 (Standards or samples) and Biotin-conjugated T4 with the pre-coated antibody specific for T4. The more amount of T4 in samples, the less antibody bound by Biotin-conjugated T4. Then Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The substrate solutions are added to the wells, respectively. And the color develops in opposite to the amount of T4 in the sample. The color development is stopped and the intensity of the color is measured.
Assay Type: Competitive
Detection Range: 20 ng/ml-320 ng/ml
Sensitivity: 10 ng/ml. Detection Wavelength: 450 nm
Sample Volume: 50-100ul
Protein Biological Process 1: Thyroid function. Pig Thyroxine, T4 ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.