Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource  rightarrow Blog  rightarrow T4 elisa kit  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Blogs moreseparator
separator
 • KLRK1 shrna blog
 • Glu-Gly-Arg-Chloromethylketone reagent blog
 • SGCZ sirna blog
 • Usnic Acid inhibitor blog
 • RWDD1 sirna blog
 • CPSF7 shrna blog
 • ZFP36L1 shrna blog
 • Tissue, Total RNA Disease, Liver Cirrhosis, Liver, BioGenomics rna blog
 • CDX2 peptide blog
 • SHB sirna blog
 • CALHM1 shrna blog
 • SMNDC1 sirna blog
 • ATG3 blocking peptide blog
 • CLDN5 elisa kit blog
 • HOMER1 sirna blog
 • EXENDIN-4 elisa kit blog
 • SMUG1 blocking peptide blog
 • TAS2R113 sirna blog
 • C19orf52 shrna blog
 • CT62 sirna blog
separator
Blog Categories separator
separator
 • Assay Kit Blogs
 • Biochemical Blogs
 • cDNA Clone Blogs
 • Monoclonal Antibody Blogs
 • Polyclonal Antibody Blogs
 • Rec/Purified Protein Blogs
 • Popular Product Blogs
separator
Our Best Sellers moreseparator
separator
 • TMPRSS2 ELISA Kit
 • Vitamin D (VD) ELISA Kit
 • STMN1 Antibody
 • Interferon Gamma (IFN-gamma) ELISA Kit
 • AKT1 - AKT Antibody
 • RXFP1 ELISA Kit
 • Advanced Glycation End Product (AGE) ELISA Kit
 • Dengue 2 full length NS1 (NS1 ) Recombinant Protein
 • Immunoglobulin G4 (IgG4) ELISA Kit
 • Vanillin 4-O- beta-D-Glucoside Biochemical
 • H3K27AC Antibody
 • IFN-gamma ELISA Kit
 • GAL12 ELISA Kit
 • HIV1 tat Antibody
 downarrow more ...
separator

T4 elisa kit product blog

separator
Posted on 2014-03-02 21:59:33 by mybiosource_staff
Share it on ...     
separator
separator
Tags: ELISA Kit; Human ELISA Kit; T4 elisa kit; thyroxine, T4;
separator
The Human T4 n/a (Catalog #MBS700320) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS700320 ELISA Kit recognizes Human (General) T4.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


(Or you can also download the PDF Manual for complete product instructions).

Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Human thyroxine, T4 ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only) T4.

Princple of the assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to T4. Standards or samples are added to the appropriate microtiter plate wells with Biotin-conjugated T4. A competitive inhibition reaction is launched between T4 (Standards or samples) and Biotin-conjugated T4 with the pre-coated antibody specific for T4. The more amount of T4 in samples, the less antibody bound by Biotin-conjugated T4. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Substrate solution is added to the wells and the color develops in opposite to the amount of T4 in the sample. The color development is stopped and the intensity of the color is measured.

Samples: Serum, plasma
Assay Type: Competitive
Detection Range: 20 ng/ml-320 ng/ml.
Sensitivity: The minimum detectable dose of human T4 is typically less than 10 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest human T4 concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<15%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<15%. Three samples of known concentration were tested in twenty assays to assess. Human thyroxine, T4 ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions