|The Fish T3 n/a (Catalog #MBS700106) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS700106 ELISA Kit recognizes Fish (Zebrafish, General) T3.
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for complete product instructions).
Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Fish Tri-iodothyronine, T3 ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
For Sample For the quantitative determination of fish tri-iodothyronine (T3) concentrations in serum, plasma and tissue homogenates.
PRINCIPLE OF THE ASSAY This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to T3. Standards or samples are added to the appropriate microtiter plate wells with Biotin-conjugated T3. A competitive inhibition reaction is launched between T3 (Standards or samples) and Biotin-conjugated T3 with the pre-coated antibody specific for T3. The more amount of T3 in samples, the less antibody bound by Biotin-conjugated T3. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Substrate solution is added to the wells and the color develops in opposite to the amount of T3 in the sample. The color development is stopped and the intensity of the color is measured.
Samples: Serum, plasma and tissue homogenates
Assay Type: Competitive
Detection Range: 0.5 ng/ml-8 ng/ml
Sensitivity: 0.249ng/ml. Detection Wavelength: 450 nm
Sample Volume: 50-100ul
Protein Biological Process 1: Thyroid function. Fish Tri-iodothyronine, T3 ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.