Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource  rightarrow Blog  rightarrow Staurosporine reagent  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Blogs moreseparator
separator
 • TMEM8C sirna blog
 • SLC39A14 sirna blog
 • NUP214 elisa kit blog
 • ZNF536 blocking peptide blog
 • SERPING1 sirna blog
 • IgG secondary antibody blog
 • XYLT2 shrna blog
 • PROSER2 sirna blog
 • Smad4 peptide blog
 • anti-TSLP antibody blog
 • PSNCA elisa kit blog
 • UGT2 elisa kit blog
 • anti-FOXM1 antibody blog
 • anti-anti-HA antibody blog
 • FOXL1 peptide blog
 • Il1rl1 recombinant protein blog
 • IL-13 elispot set blog
 • anti-c-Abl (Tyr-412) antibody blog
 • Vapreotide peptide blog
 • anti-17-HYDROXYPREGNENOLONE antibody blog
separator
Blog Categories separator
separator
 • Assay Kit Blogs
 • Biochemical Blogs
 • cDNA Clone Blogs
 • Monoclonal Antibody Blogs
 • Polyclonal Antibody Blogs
 • Rec/Purified Protein Blogs
 • Popular Product Blogs
separator
Our Best Sellers moreseparator
separator
 • Reduced Glutathione (GSH) ELISA Kit
 • MAPK14 Antibody
 • A Disintegrin and Metalloprotease 20 (ADAM20) ELISA Kit
 • CYP2E1 ELISA Kit
 • EFEMP2 Antibody
 • Interleukin-11 (IL 11) Recombinant Protein
 • Creatinine Acid Antibody
 • S100 calcium binding protein A1 (S100A1) ELISA Kit
 • Renin Antibody
 • growth differentiation factor 2 (GDF2) ELISA Kit
 • Metallothionein-2 (MT-2) ELISA Kit
 • Chorionic somatomammotropin hormone 1 (CSH1) ELISA Kit
 • Advanced Glycosylation End Products (AGEs) ELISA Kit
 • CCNB1 siRNA
 downarrow more ...
separator

Staurosporine reagent product blog

separator
Posted on 2018-06-07 04:17:04 by mybiosource_staff
Share it on ...     
separator
separator
Tags: Reagent; Staurosporine; Staurosporine reagent;
separator
The Staurosporine n/a (Catalog #MBS258055) is a Reagent and is intended for research purposes only. The product is available for immediate purchase.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


Staurosporine is a natural product derived alkaloid which is isolated from Streptomyces staurospores cultures. This protein kinase inhibitor belongs to a family of kinase inhibitors containing an indole carbozol chromophore and is a potent inhibitor of a number of kinases. The ATP binding site on these kinase enzymes appears to be targeted by these Streptomyces derived inhibitors.

Inhibition of these intracellular kinases leads to the induction of apoptosis as exhibited by the classic chromatin condensation leading to the formation of micronuclear bodies and reduced cell volumes. DNA is subsequently cleaved into oligonucleosomal fragments (laddering) as evidenced by agarose gel analysis.

Staurosporine can be used with ICTís apoptosis and cytotoxicity detection assays to create positive controls.

Staurosporine n/a

Protocol: 1. Dissolve staurosporine powder in tissue culture grade DMSO to obtain a 1 mM staurosporine concentration.
2. Prepare 50 - 100 uL aliquots of the DMSO solubilized staurosporine stock solution and store them frozen at < -20 degree C. A frozen vial of staurosporine may only be rethawed 2X before it must be discarded.
3. Spike cell cultures at a staurosporine concentration of 1 uM in the cell culture media. This equates to a 1 uL spike of the 1 mM staurosporine stock per mL of the cell culture suspension. This concentration works well for inducing many cell lines including HL-60 and Jurkat cells when using a 4-5 hour 37 degree C incubation period. Typical cell suspension concentrations that have been used for this staurosporine induction protocol range from 1 x 10^5 - 1 x 10^6 cells/mL.
4. Perform time course studies on your particular cell line to ascertain the optimal staurosporine concentration and exposure time required to achieve good apoptosis induction levels in your experimental system.
5. Proceed with your experimental apoptosis induction model system.
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions