Spleen lysate product blog
Tags: Lysate; Spleen; Spleen lysate;
The Spleen n/a (Catalog #MBS151674) is a Lysate produced from Human and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s Spleen can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the Spleen n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Spleen tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 mug/ml of aprotinin, 5 mug/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.
Species: Human
Source: Human spleen tissue
Buffer: Spleen lysate is supplied in SDS sample buffer containing 5% Beta-mercaptoethanol.
By Type: Tissue. Tissue/Cell Type: Spleen
Tissue by Dagnosis: Normal
Identification: Normal
Tissue by Species: Human.