sP53 elisa kit product blog
Tags: ELISA Kit; Rabbit ELISA Kit; Soluble P53; sP53 elisa kit; sP53;
The Rabbit sP53 n/a (Catalog #MBS721689) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS721689 ELISA Kit recognizes Rabbit sP53.To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Rabbit Soluble P53 ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
For samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
INTENDED USE This sP53 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rabbit sP53. This ELISA kit for research use only!
PRINCIPLE OF THE ASSAY sP53 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-sP53 antibody and an sP53-HRP conjugate. The assay sample and buffer are incubated together with sP53-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the sP53 concentration since sP53 from samples and sP53-HRP conjugate compete for the anti-sP53 antibody binding site. Since the number of sites is limited, as more sites are occupied by sP53 from the sample, fewer sites are left to bind sP53-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The sP53 concentration in each sample is interpolated from this standard curve.
Samples: Serum, plasma, cell culture supern atants, body fluid and tissue homogenate
Assay Type: Competitive
Sensitivity: 0.1 ng/mL. Rabbit Soluble P53 ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.