SOD3 elisa kit product blog
Tags: ELISA Kit; Human ELISA Kit; SOD3 elisa kit; SOD3;
The Human SOD3 sod3 (Catalog #MBS2514105) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS2514105 ELISA Kit recognizes Human SOD3.The SOD3 sod3 product has the following accession number(s) (GI #49456661) (NCBI Accession #CAG46651.1) (Uniprot Accession #P08294). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Human SOD3 (Superoxide Dismutase 3, Extracellular) ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human SOD3 concentrations in serum, plasma and other biological fluids.
Principle of the Assay: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Human SOD3. During the reaction, Human SOD3 in the sample or standard competes with a fixed amount of Human SOD3 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human SOD3. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme- substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Human SOD3 in the samples is then determined by comparing the OD of the samples to the standard curve.
Samples: Serum, plasma and other biological fluids
Assay Type: Quantitative Competitive
Detection Range: 6.25-400ng/mL
Sensitivity: 3.75ng/mL. Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human SOD3 were tested 20 times on one plate, respectively.
Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human SOD3 were tested on 3 different plates, 20 replicates in each plate. Human SOD3 (Superoxide Dismutase 3, Extracellular) ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit. Cardiovascular Diseases, Disease Models, Animal, Disease Progression, Heart Diseases, Hypertension, Inflammation, Lung Diseases, Lung Injury, Nervous System Diseases, Pulmonary Disease, Chronic Obstructive are some of the diseases may be linked to Human SOD3 (Superoxide Dismutase 3, Extracellular) ELISA Kit.