Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource  rightarrow Blog  rightarrow SIgA elisa kit  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Blogs moreseparator
separator
 • TFEC blocking peptide blog
 • anti-Apo CIII antibody blog
 • anti-IGFL3 antibody blog
 • AWRI1631_142870 blocking peptide blog
 • anti-IFNA3 antibody blog
 • anti-PKCD antibody blog
 • phrB recombinant protein blog
 • anti-HRH1 antibody blog
 • anti-JAGGED2 antibody blog
 • EDN3 native protein blog
 • RBM15B shrna blog
 • Datura stramonium lectin blog
 • SPIC shrna blog
 • ERK2 elisa kit blog
 • AGBL4 blocking peptide blog
 • HMGCL elisa kit blog
 • LRWD1 sirna blog
 • TM7SF2 sirna blog
 • HSPA1A antibody pair blog
 • TAS2R119 sirna blog
separator
Blog Categories separator
separator
 • Assay Kit Blogs
 • Biochemical Blogs
 • cDNA Clone Blogs
 • Monoclonal Antibody Blogs
 • Polyclonal Antibody Blogs
 • Rec/Purified Protein Blogs
 • Popular Product Blogs
separator
Our Best Sellers moreseparator
separator
 • Vacuolar Protein Sorting 28 (VPS28) Recombinant Protein
 • ADAMTS-like 2 (ADAMTSL2) ELISA Kit
 • Cyclin-dependent kinase 20 (CCRK) ELISA Kit
 • Apolipoprotein C3 (APOC3) Antibody
 • Mammaglobin A - SCGB2A2 Peptide
 • adenylate kinase 3 (AK3) ELISA Kit
 • ZBTB16 Antibody
 • Bcl-2 ELISA Kit
 • Leucine-rich repeat flightless-interacting protein 1 (LRRFIP... Antibody
 • proteinkinaseC (PKC) ELISA Kit
 • CD226 Blocking Peptide
 • Interleukin 8 (IL8) Recombinant Protein
 • Herpes Simplex Virus Type 1 & 2 (HSV 1&2) Glycoprotein D Antibody
 • Caspase-8 (CASP8) Antibody
 downarrow more ...
separator

SIgA elisa kit product blog

separator
Posted on 2014-02-21 02:12:39 by mybiosource_staff
Share it on ...     
separator
separator
Tags: ELISA Kit; Secretory Immunoglobulin A; SIgA elisa kit; SIgA; Guinea Pig ELISA Kit;
separator
The Guinea Pig SIgA n/a (Catalog #MBS720392) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS720392 ELISA Kit recognizes Guinea Pig (Cavia, Cavy, Porcellus, General) SIgA.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


(Or you can also download the PDF Manual for complete product instructions).

Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Guinea pig Secretory Immunoglobulin An ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only) SIgA.

Intended Uses: This sIgA ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Guinea sIgA. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: sIgA ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for sIgA. Standards or samples are then added to the microtiter plate wells and sIgA if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of sIgA present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for sIgA are added to each well to "sandwich" the sIgA immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain sIgA and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The sIgA concentration in each sample is interpolated from this standard curve.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type: Sandwich
Detection Range: 5.0-100mg/mL
Sensitivity: 1.0mg/mL. Guinea pig Secretory Immunoglobulin An ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions