|The SERPINA1 serpina1 (Catalog #MBS564064) is an ELISA Kit produced from Mouse and is intended for research purposes only. The product is available for immediate purchase.
The SERPINA1 serpina1 product has the following accession number(s) (GI #177831) (Uniprot Accession #P01009). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
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Principle of the Assay: The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the A1AT present in samples reacts with the anti-A1AT antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-A1AT antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound A1AT. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3\',5,5\'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of A1AT in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of A1AT in the test sample. The quantity of A1AT in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
Background: Alpha 1-Antitrypsin is a 52 kDA serine protease inhibitor (serpin) in blood, which protects tissue from enzymes from inflammatory cells, especially elastase. In certain acute phase inflammatory reactions, A1AT is elevated in order to limit the damage caused by activated neutrophil granulocytes and their enzyme elastase. Disorders of A1AT include A1AT deficiency, a hereditary disorder that can lead to severe tissue breakdown during inflammation. This may result in pulmonary emphysema and liver cirrhosis, in severe cases. Genetic variants of A1AT do occur.
Samples: Immunoperoxidase Assay for Determination of Alpha 1-Antitrypsin in Mouse Samples
Assay Type: Sandwich. Intended Uses: The Alpha 1-Antitrypsin (A1AT) test kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring A1AT in biological samples of mice. Mouse Alpha 1-Antitrypsin ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit. SERPINA1 also interacts with the following gene(s): CTSG, PRTN3. The following patways have been known to be associated with this gene.