Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
Fax 1.858.633.0166    Email 
menu1 Home Products Ordering Company Help menu7
   MyBioSource  rightarrow Blog  rightarrow SERPINA1 elisa kit  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Blogs moreseparator
 • ZNF608 sirna blog
 • 200 kDa Neurofilament native protein blog
 • Goat Anti-Human IgG (H&L) FITC secondary antibody blog
 • COMT sirna blog
 • anti-ATG7 antibody blog
 • CCDC7 sirna blog
 • Creatine Kinase MM protein blog
 • anti-Methamphetamine antibody blog
 • anti-AKR1C3 antibody blog
 • anti-CD49b, VLA alpha-2 chain (Collagen Receptor) antibody blog
 • Mouse Anti-Mouse I-A^b secondary antibody blog
 • MAPT blocking peptide blog
 • PLA2G10 elisa kit blog
 • Micafungin sodium inhibitor blog
 • ATAT1 sirna blog
 • ADAM10 elisa kit blog
 • SPATA22 shrna blog
 • anti-ASIALO GM1 antibody blog
 • anti-NT5E antibody blog
 • CORT elisa kit blog
Blog Categories separator
 • Assay Kit Blogs
 • Biochemical Blogs
 • cDNA Clone Blogs
 • Monoclonal Antibody Blogs
 • Polyclonal Antibody Blogs
 • Rec/Purified Protein Blogs
 • Popular Product Blogs
Our Best Sellers moreseparator
 • ApoC4 ELISA Kit
 • SIRT1 Antibody
 • solute carrier family 30 (zinc transporter) , member 2 (SLC3... ELISA Kit
 • C-C chemokine receptor type 9 (CCR9) Recombinant Protein
 • N-acetylglucosamine Antibody
 • Albumin Antibody
 • Phosphoenolpyruvate Carboxykinase 1, Soluble (PCK1) ELISA Kit
 • Elastase, Neutrophil Recombinant Protein
 • SHP-1 Antibody
 • Urea ELISA Kit
 • S3-12-KIAA1881 Antibody
 • interleukin 24 (IL24) ELISA Kit
 • Ovomucoid Antibody
 downarrow more ...

SERPINA1 elisa kit product blog

Posted on 2015-06-05 15:19:04 by mybiosource_staff
Share it on ...     
Tags: ELISA Kit; SERPINA1; Alpha 1-Antitrypsin; SERPINA1 elisa kit;
The SERPINA1 serpina1 (Catalog #MBS564064) is an ELISA Kit produced from Mouse and is intended for research purposes only. The product is available for immediate purchase.

The SERPINA1 serpina1 product has the following accession number(s) (GI #177831) (Uniprot Accession #P01009). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

(Or you can also download the PDF Manual for complete product instructions).

Principle of the Assay: The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the A1AT present in samples reacts with the anti-A1AT antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-A1AT antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound A1AT. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3\',5,5\'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of A1AT in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of A1AT in the test sample. The quantity of A1AT in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

Background: Alpha 1-Antitrypsin is a 52 kDA serine protease inhibitor (serpin) in blood, which protects tissue from enzymes from inflammatory cells, especially elastase. In certain acute phase inflammatory reactions, A1AT is elevated in order to limit the damage caused by activated neutrophil granulocytes and their enzyme elastase. Disorders of A1AT include A1AT deficiency, a hereditary disorder that can lead to severe tissue breakdown during inflammation. This may result in pulmonary emphysema and liver cirrhosis, in severe cases. Genetic variants of A1AT do occur.

SERPINA1 serpina1

Samples: Immunoperoxidase Assay for Determination of Alpha 1-Antitrypsin in Mouse Samples
Assay Type: Sandwich. Intended Uses: The Alpha 1-Antitrypsin (A1AT) test kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring A1AT in biological samples of mice. Mouse Alpha 1-Antitrypsin ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit. The following patways have been known to be associated with this gene. Blood, Bone, Heart, Intestine, Kidney, Liver, Lung, Pancreas, Skin, Vascular tissues are correlated with this protein.
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions