SEMA3F elisa kit product blog
Tags: ELISA Kit; Human ELISA Kit; SEMA3F elisa kit; SEMA3F; Semaphorin 3F (SEMA3F);
The Human SEMA3F sema3f (Catalog #MBS161003) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS161003 ELISA Kit recognizes Human SEMA3F.The SEMA3F sema3f product has the following accession number(s) (GI #188497703) (NCBI Accession #NP_004177.3) (Uniprot Accession #Q13275). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Human Semaphorin 3F (SEMA3F) ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Intended Uses: This sandwich kit is for the accurate quantitative detection of human Semaphorin 3F (also known as SEMA3F) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.
Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with human SEMA3F antibody. SEMA3F present in the sample is added and binds to antibodies coated on the wells. And then biotinylated human SEMA3F Antibody is added and binds to SEMA3F in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated SEMA3F antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of human SEMA3F. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Samples: Serum, plasma, cell culture supernates, cell lysates, tissue homogenates
Assay Type: Quantitative Sandwich
Detection Range: 0.1ng/ml - 40ng/ml
Sensitivity: 0.05ng/ml. Intra-Assay: CV<8%
Inter-Assay: CV<10%. Human Semaphorin 3F (SEMA3F) ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit. Bone, Brain, Embryonic Tissue, Eye, Heart, Intestine, Kidney, Lung, Mouth, Ovary tissues are correlated with this protein. Adenocarcinoma, Breast Neoplasms, Cardiovascular Diseases, Disease Models, Animal, Inflammation, Lung Neoplasms, Myocardial Infarction, Neoplasms, Ovarian Diseases, Stroke are some of the diseases may be linked to Human Semaphorin 3F (SEMA3F) ELISA Kit. The following patways have been known to be associated with this gene. SEMA3F also interacts with the following gene(s): NRP1, NRP2, PLXNA1, PLXNA3, VEGFA, ZEB1.