Scd14 elisa kit product blog
Tags: ELISA Kit; Sheep ELISA Kit; sCD14; SCD14 elisa kit;
The Sheep Scd14 n/a (Catalog #MBS734472) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS734472 ELISA Kit recognizes Sheep Scd14.To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Sheep sCD14 ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Intended Uses: This sCD14 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Sheep sCD14. This ELISA kit for research use only!
Principle of the Assay: sCD14 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-sCD14 antibody and an sCD14-HRP conjugate. The assay sample and buffer are incubated together with sCD14-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the sCD14 concentration since sCD14 from samples and sCD14-HRP conjugate compete for the anti-sCD14 antibody binding site. Since the number of sites is limited, as more sites are occupied by sCD14 from the sample, fewer sites are left to bind sCD14-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The sCD14 concentration in each sample is interpolated from this standard curve.
Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type: Quantitative Competitive
Sensitivity: 1.0 ng/mL. Sheep sCD14 ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.