|The Sambucus nigra n/a (Catalog #MBS656494) is a Lectin and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s Sambucus nigra can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC).
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Researchers should empirically determine the suitability of the Sambucus nigra n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
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This lectin is a 150kD tetrameric glycoprotein consisting of four disulfide-linked chains, two of about 36kD and two of about 38kD. Sambucus nigra lectin binds preferentially to sialic acid attached to terminal galactose in (a-2,6), and to a lesser degree, (a-2,3), linkage. Binding is also inhibited to some extent by lactose or galactose. This lectin does not appear to bind sialic acid linked to N-acetylgalacto-samine. SNA has been reported to inhibit cell-free protein synthesis. This biotinylated lectin conjugate is prepared from affinity-purified lectin and is optimally labeled with biotin. Essentially free of inactive lectin conjugate and containing no free biotin, this biotinylated lectin provides an ideal intermediate for examining glycoconjugates using the Biotin-Avidin System. First the biotin-labeled lectin is added, followed by an ABC Reagent, Avidin D conjugate, or streptavidin derivative. Another possible application is in the isolation of lymphokines and other products of mitogenic stimulation.