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Sambucus nigra lectin product blog

Posted on 2014-10-06 13:05:36 by mybiosource_staff
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Tags: Lectin; Sambucus nigra; Sambucus nigra lectin;
The Sambucus nigra n/a (Catalog #MBS656355) is a Lectin and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s Sambucus nigra can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA).
Suitable for use in ELISA. Researchers should empirically determine the suitability of the Sambucus nigra n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

This lectin is a 150,000 molecular weight tetrameric glycoprotein consisting of four disulfide-linked chains, two of about 36,000D and two of about 38,000D. Sambucus nigra lectin binds preferentially to sialic acid attached to terminal galactose in (a-2,6), and to a lesser degree, (a-2,3), linkage. Binding is also inhibited to some extent by lactose or galactose. This lectin does not appear to bind sialic acid linked to N-acetylgalactosamine. SNA has been reported to inhibit cell-free protein synthesis. Fluorescein labeled Sambucus Nigra Lectin is produced by using the highest quality fluorescein isothiocyanate, our affinity-purified lectin, and special conjugation procedures. Fluorescein labeled Sambucus Nigra Lectin has an appropriate number of fluorochromes bound which provide the maximum fluorescence and optimum staining characteristics for this particular lectin. This lectin is supplied essentially free of unconjugated fluorochromes and inactive lectin. Accompanying each fluorescent lectin is an analysis data sheet summarizing the results of our quality control tests and providing pertinent information on the product. All of these reagents are supplied as solutions perserved with sodium azide.

Sambucus nigra n/a

Emission: 515nm
Excitation: 495nm
F/P (molar): 6.4
Recommended Dilution: 2-20ug/ml. Optimal dilutions to be determined by the researcher.
Elution: 500mM lactose in buffered saline followed by 500mM lactose in acetic acid.: F/P(molar): 6.4
Active conjugate: 97%.
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