Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
Fax 1.858.633.0166    Email 
menu1 Home Products Ordering Company Help menu7
   MyBioSource  rightarrow Blog  rightarrow SA elisa kit  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Blogs moreseparator
 • platelet antibodies IgM (PA-IgM) elisa kit blog
 • anti-APOE antibody blog
 • CFL1 blocking peptide blog
 • ENO2 elisa kit blog
 • RHOQ blocking peptide blog
 • HTLV-I elisa kit blog
 • Gamma Globulin, Cohn Fraction II, Bovine immunoglobulin blog
 • SEC23IP sirna blog
 • MAGEH1 sirna blog
 • FAM3D elisa kit blog
 • anti-PTC1 antibody blog
 • Calcineurin Autoinhibitory peptide blog
 • RRNAD1 sirna blog
 • SLITRK3 shrna blog
 • CLSPN sirna blog
 • IgG secondary antibody blog
 • anti-FSH antibody blog
 • UCHL1 antibody pair blog
 • ACTC1 sirna blog
 • CRP purified protein blog
Blog Categories separator
 • Assay Kit Blogs
 • Biochemical Blogs
 • cDNA Clone Blogs
 • Monoclonal Antibody Blogs
 • Polyclonal Antibody Blogs
 • Rec/Purified Protein Blogs
 • Popular Product Blogs
Our Best Sellers moreseparator
 • Natural Cytotoxicity Receptor NKp46 (NKp46 ) Recombinant Protein
 • Transthyretin (TTR) Recombinant Protein
 • Adrenomedullin (ADM) Antibody
 • Anti-Complement 1q antibody (anti-C1q-antibody) ELISA Kit
 • Actin-alpha-1 (ACTA1) Antibody
 • KPNA1-Importin alpha 5 Antibody
 • Calbindin (CALB1) ELISA Kit
 • Growth factor receptor-bound protein 10 (Grb10) Recombinant Protein
 • activating transcription factor 6 beta (ATF6B) Antibody
 • Defensins Alpha 2 (DEFA2) ELISA Kit
 • RB1 cDNA Clone
 • Interleukin 15 (IL15) Antibody
 • Norrie disease (pseudoglioma) (NDP) ELISA Kit
 downarrow more ...

SA elisa kit product blog

Posted on 2013-08-02 18:17:26 by mybiosource_staff
Share it on ...     
Tags: ELISA Kit; Mouse ELISA Kit; Sialic Acid; SA elisa kit;
The Mouse SA n/a (Catalog #MBS729807) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS729807 ELISA Kit recognizes Mouse (Murine, General) SA.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

(Or you can also download the PDF Manual for complete product instructions).

Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Mouse Sialic acid ELISA Kit with the following immunoassay(s):
Typical Standard Curve/Testing Data
Typical Standard Curve/Testing Data SA.

Intended Uses: This SA ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse SA. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: SA ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-SA antibody and an SA-HRP conjugate. The assay sample and buffer are incubated together with SA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SA concentration since SA from samples and SA-HRP conjugate compete for the anti-SA antibody binding site. Since the number of sites is limited, as more sites are occupied by SA from the sample, fewer sites are left to bind SA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SA concentration in each sample is interpolated from this standard curve.

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type: Quantitative Competitive
Sensitivity: 0.1 mg/L. Mouse Sialic acid ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions