Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
Fax 1.858.633.0166    Email 
menu1 Home Products Ordering Company Help menu7
   MyBioSource  rightarrow Blog  rightarrow SA elisa kit  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Blogs moreseparator
 • DENV-Env antigen blog
 • anti-TAAR5 antibody blog
 • RNF152 sirna blog
 • TNFAIP3 shrna blog
 • PNPLA3 positive control blog
 • PTPRO sirna blog
 • EV71 elisa kit blog
 • LRP10 sirna blog
 • SMTNL1 blocking peptide blog
 • Tissue, Section, Monkey tissue section blog
 • Epidermal Growth Factor [des-Leu26, Cys(Acm)20,31] peptide blog
 • BP-1 (Ly-51, 6C3) secondary antibody blog
 • IQCJ sirna blog
 • MEIG1 shrna blog
 • anti-MMP-23 antibody blog
 • anti-CDH2 antibody blog
 • anti-CD40 antibody blog
 • CD4 peptide blog
 • sP-Selectin elisa kit blog
 • CHD8 sirna blog
Blog Categories separator
 • Assay Kit Blogs
 • Biochemical Blogs
 • cDNA Clone Blogs
 • Monoclonal Antibody Blogs
 • Polyclonal Antibody Blogs
 • Rec/Purified Protein Blogs
 • Popular Product Blogs
Our Best Sellers moreseparator
 • DBI Antibody
 • cdc34 Antibody
 • Lactoperoxidase (LPO ) Antibody
 • p140Cap Antibody
 • Hemoglobin subunit gamma-1 (HBG1) Antibody
 • sCD14 ELISA Kit
 • microtubule-associated protein 1 light chain 3 beta (MAP1LC3... ELISA Kit
 • Aquaporin 2, Collecting Duct (AQP 2) ELISA Kit
 • Osteonectin (SPARC) Native Protein
 • Calgranulin A Antibody
 • SPP1 Antibody
 • NT-proBNP ELISA Kit
 • S-formylglutathione hydrolase (Esd) Recombinant Protein
 • KIF26B Antibody
 downarrow more ...

SA elisa kit product blog

Posted on 2013-08-02 18:17:26 by mybiosource_staff
Share it on ...     
Tags: ELISA Kit; Mouse ELISA Kit; Sialic Acid; SA elisa kit;
The Mouse SA n/a (Catalog #MBS729807) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS729807 ELISA Kit recognizes Mouse (Murine, General) SA.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

(Or you can also download the PDF Manual for complete product instructions).

Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Mouse Sialic acid ELISA Kit with the following immunoassay(s):
Typical Standard Curve/Testing Data
Typical Standard Curve/Testing Data SA.

Intended Uses: This SA ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse SA. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: SA ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-SA antibody and an SA-HRP conjugate. The assay sample and buffer are incubated together with SA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SA concentration since SA from samples and SA-HRP conjugate compete for the anti-SA antibody binding site. Since the number of sites is limited, as more sites are occupied by SA from the sample, fewer sites are left to bind SA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SA concentration in each sample is interpolated from this standard curve.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type: Sandwich
Detection Range: 0.5-10ng/mL
Sensitivity: 0.1ng/mL. Mouse Sialic acid ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions