Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource  rightarrow Blog  rightarrow S100A12 elisa kit  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Blogs moreseparator
separator
 • SYNJ1 blocking peptide blog
 • ABTB2 sirna blog
 • anti-HIST1H3A antibody blog
 • anti-KIR2DL5B antibody blog
 • TRAFD1 shrna blog
 • TMED4 sirna blog
 • PPM1B sirna blog
 • SFSWAP shrna blog
 • SLC36A3 shrna blog
 • Oligo, ssAdapter, BamH I- Sma I adapter blog
 • CSF1 elisa kit blog
 • STX4 shrna blog
 • anti-CD155 antibody blog
 • C5a elisa kit blog
 • entC3 recombinant protein blog
 • anti-PIWIL1/MIWI antibody blog
 • TCF12 blocking peptide blog
 • ERC1 sirna blog
 • TAS2R38 shrna blog
 • PLK1 peptide blog
separator
Blog Categories separator
separator
 • Assay Kit Blogs
 • Biochemical Blogs
 • cDNA Clone Blogs
 • Monoclonal Antibody Blogs
 • Polyclonal Antibody Blogs
 • Rec/Purified Protein Blogs
 • Popular Product Blogs
separator
Our Best Sellers moreseparator
separator
 • PTPN6 ELISA Kit
 • Glutenin, high molecular weight subunit DX5 (GLU-1D-1D) Recombinant Protein
 • CD25 (IL2RA) Antibody
 • Interferon beta-1 (IFNB1) ELISA Kit
 • Tyrosinase Assay Kit
 • Interferon-gamma Receptor alpha chain (Ab-457) (IFNGR1) Antibody
 • V-set and immunoglobulin domain-containing protein 10 (Vsig1... Recombinant Protein
 • Deoxyribonuclease gamma (Dnase1l3) Recombinant Protein
 • CD151 cDNA Clone
 • ATPase inhibitor, mitochondrial (ATPIF1) ELISA Kit
 • A2M-alpha2-Macroglobulin ELISA Kit
 • Mitogen-Activated Protein Kinase 3 (MAPK3) Recombinant Protein
 • Escherichia coli (cfaE) Recombinant Protein
 • PLTP Antibody
 downarrow more ...
separator

S100A12 elisa kit product blog

separator
Posted on 2013-10-11 12:52:13 by mybiosource_staff
Share it on ...     
separator
separator
Tags: ELISA Kit; Mouse ELISA Kit; S100 Calcium Binding Protein A12; S100A12 elisa kit; S100A12;
separator
The Mouse S100A12 s100a12 (Catalog #MBS728991) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS728991 ELISA Kit recognizes Mouse (Murine, General) S100A12.

The S100A12 s100a12 product has the following accession number(s) (GI #119573717) (NCBI Accession #EAW53332.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


(Or you can also download the PDF Manual for complete product instructions).

Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Mouse S100 Calcium Binding Protein A12 ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve
Typical Testing Data/Standard Curve S100A12.

Intended Uses: This S100A12 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse S100A12. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: S100A12 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for S100A12. Standards or samples are then added to the microtiter plate wells and S100A12 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of S100A12 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for S100A12 are added to each well to "sandwich" the S100A12 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain S100A12 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The S100A12 concentration in each sample is interpolated from this standard curve.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type: Sandwich
Detection Range: 50-1000pg/mL
Sensitivity: 1.0pg/mL. Mouse S100 Calcium Binding Protein A12 ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions