|The Human rT3 n/a (Catalog #MBS701460) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS701460 ELISA Kit recognizes Human (General) rT3.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual
for complete product instructions).
Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Human ReverseTri-iodothyronine, rT3 ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. An antibody specific to rT3 has been pre-coated onto a microplate. Standards or samples are added to the appropriate microtiter plate wells with biotin-conjugated rT3 and incubated. A competitive inhibition reaction is launched between rT3 (Standards or samples) and Biotin-conjugated rT3 with the pre-coated antibody specific for rT3. The more amount of rT3 in samples, the less antibody bound by Biotin-conjugated T3. Then Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The substrate solutions are added to the wells, respectively. And the color develops in opposite to the amount of rT3 in the sample. The color development is stopped and the intensity of the color is measured.
Samples: Serum, plasma, cell culture supernates and tissue homogenates
Assay Type: Competitive
Detection Range: 75 pg/ml-1200 pg/ml
Sensitivity: 37.5pg/ml. Detection Wavelength: 450 nm
Sample Volume: 50-100ul. Human ReverseTri-iodothyronine, rT3 ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.