RPMI 1640 Medium Modified w/o L-Glutamine, w/o Amino acids, Glucose, Phenol Red culture media product blog
Tags: Culture Media; RPMI 1640 Medium Modified w/o L-Glutamine, w/o Amino acids, Glucose, Phenol Red; RPMI 1640 Medium Modified w/o L-Glutamine, w/o Amino acids, Glucose, Phenol Red culture media;
The RPMI 1640 Medium Modified w/o L-Glutamine, w/o Amino acids, Glucose, Phenol Red n/a (Catalog #MBS653421) is a Culture Media and is intended for research purposes only. The product is available for immediate purchase.To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
RPMI 1640 is used for the culture of human normal and neoplastic leukocytes. RPMI 1640 was developed by Moore et al. at Roswell Park Memorial Institute.
Appearance: Light yellow, homogeneous, free flowing powder.
Solubility: Colorless, clear, complete
pH: 8.1
Endotoxin: < 1 EU/ml. Directions per Liter: Dissolve 7.4g in 800-900mL of ddH2O, stirring gently until completely solubilized. Do NOT heat. If required, add 2g sodium bicarbonate with stirring. Adjust pH of the medioum to 0.1-0.3 pH unit below the desired pH. Add additional water to bring the solution to 1L. Filter-sterilize using a 0.22 um membrane. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.
Inorganic Salts: Calcium Nitrate�4H2O - 0.1
Magnesium Sulfate - 0.04884
Potassium Chloride - 0.4
Sodium Chloride - 6
Sodium Phosphate Dibasic - 0.8
Amino Acids: L-Arginine - Absent
L-Aspartic Acid - Absent
L-Asparagine - Absent
L-Cystine�2HCl - Absent
L-Glutamic Acid - Absent
L-Glutamine - Absent
Glycine - Absent
L-Histidine - Absent
Hydroxy-L-proline - Absent
L-Isoleucine - Absent
L-Leucine - Absent
L-Lysine�HCl - Absent
L-Methionine - Absent
L-Phenylalanine - Absent
L-Proline - Absent
L-Serine - Absent
L-Threonine - Absent
L-Tryptophan - Absent
L-Tyrosine�2Na�2H2O - Absent
L-Valine - Absent
Vitamins: P-Amino Benzoic Acid - 0.001
D-Biotin - 0.0002
Choline Chloride - 0.003
Vitamin B-12 - 0.000005
Folic Acid - 0.001
myo-Inositol - 0.035
Niacinamide - 0.001
D-Pantothenic Acid�Ca - 0.00025
Pyridoxine�HCl - 0.001
Riboflavin - 0.0002
Thiamine�HCl - 0.001
Other: Glucose - Absent
Glutathione - 0.001
Phenol Red, Sodium - Absent
Total: 7.4g/liter.