RNA Marker marker product blog
Tags: Marker; RNA Marker; RNA Marker marker;
The RNA Marker n/a (Catalog #MBS657635) is a Marker and is intended for research purposes only. The product is available for immediate purchase.To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given marker. We\'ve tested the RNA Marker (Ladder), Low Range with the following immunoassay(s):
Testing Data (RNA Marker (Ladder), Low Range (100-1000bp))
The RNA Ladder, Low Range is a mixture of seven chromatography-purified single-stranded RNA transcripts. Their lengths are (in bases): 1000, 800, 600, 400, 300, 200 and 100. The ladder is designed for qualitative and quantitative analysis of RNA on agarose gels stained with ethidium bromide or SYBR® Green II. The ladder is free of degraded RNA and NTP\'s. Therefore, spectrophotometric measurements provide accurate values of RNA concentration in each ladder band (see photos for quantitative data). Due to this feature, the RNA ladder could be used for approximate RNA quantification on gels.
Note: 1. When loading on gel use the same 2X Loading Dye Solution to both your RNA sample and RNA ladder.
2. When visualizing a gel in UV light, additional dark zone of ethidium bromide sometimes may be observed. It appears due to mobility of ethidium bromide (in direction opposite to that of RNA) during electrophoresis. This, however, has no influence on the quality of RNA separation.
3. For optimal results, add ethidium bromide to both native agarose gel and electrophoresis buffer at the same final concentration of 0.5ug/ml. Run gels at 5V/cm.
4. After electrophoresis in polyacrylamide gel, RNA Ladder should be stained with ethidium bromide at a concentration of 0.5ug/ml for 20 min.
Storage Buffer: 1mM EDTA (pH 6.0).