Raf-1 RBD assay reagent product blog
Tags: Assay Reagent; Raf-1 RBD; Raf-1 RBD assay reagent;
The Raf-1 RBD n/a (Catalog #MBS169168) is an Accessory Reagent and is intended for research purposes only. The product is available for immediate purchase.To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given accessory reagent. We\'ve tested the Raf-1 RBD Agarose Beads with the following immunoassay(s):
Testing Data
• Pull down the active form of Ras
• Colored for easy visualization
Small GTP-binding proteins (or GTPases) are a family of proteins that serve as molecular regulators in signaling transduction pathways. Ras, a 21 kDa protein, regulating a variety of biological response pathways that include cell growth, cell transformation and tumor invasion. Like other small GTPases, Ras regulates molecular events by cycling between an inactive GDP-bound form and an active GTPbound form. In its active (GTP-bound) state, Ras binds specifically to the Ras-binding domain (RBD) of Raf1 to control downstream signaling cascades.
Activity: Product specifically interacts and precipitaes GTP-bound Ras from cell lysate. Quantity: 800 uL of 50% Agarose slurry, 400 ug Raf1-RBD in 1X PBS,50% Glycerol.